Page 63 - Applied Pharmacognosy
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Classification of Chromatographic Techniques
According to the Mode of Retention
3. Ion-Exchange Chromatography.
✓ Stationary phase: Insoluble polymer or resin having a charge
bearing functional group (covalently linked).
✓ Mobile phase: Liquid.
✓ Used for separation of ionized substances based on their
affinity to the ion exchanger.
Ion-Exchanger Stationary Phase Sample
Anion exchanger R----N+---- Cl- Phenolic sub.-
Cation exchanger R----SO3------H+
Alkaloid +
Sample Stationary phase
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