Page 11 - Biochemistry PharmD General

Phosphofructokinase (PFK-1) as a
regulator of glycolysis

fructose-6-phosphate PFK-1
fructose-1,6-bisphosphate

PFK allosterically inhibited by:

• High ATP lower affinity for

fructose-6-phosphate by binding to a

regulatory site distinct from catalytic

site.

• High H+ reduced activity to prevent

excessive lactic acid formation and drop in

blood pH (acidosis).

• Citrate prevents glycolysis by

accumulation of this citric acid cycle

intermediate to signal ample biosynthetic

precursors and availability of fatty acids

or ketone bodies for oxidation. 10