Page 4 - Instrumental Analysis - Pharm D Clinical- 07-PA403

UV-visible Spectrophotometry

Spectroscopic methods of analysis

• Spectroscopy is the science dealing with the interactions of
radiation and matter.

• Measurements based on light and other forms of electromagnetic
radiation are widely used throughout analytical chemistry.

• Spectroscopic analytical methods are based on measuring the
amount of radiation absorbed or produced by the analyte.

• This course deals with 2 types of spectroscopic methods of analysis:
1- Molecular absorption spectroscopy
2- Molecular emission (fluorescence) spectroscopy

3

Electromagnetic Radiation

• Electromagnetic radiation is a form of energy that consists of
perpendicular oscillating electric and magnetic fields that
propagate through space along a linear path and with constant
velocity (3 x 108 m/sec).

• We call electromagnetic radiation in the UV/visible and sometimes
in the IR region, light.

4

3