Page 47 - 01 CARBOHYDRATES for TCM
P. 47

Phenol-sulphuric Acid






     Method to determine total polysaaccharides






    Material & instruments

        Sulfuric acid (AR grade)

        Phenol (AR grade)

        Glucose (Ref STD)
        Microplate reader

        96 wells microplate flat bottom

        Micro pipette


      Glucose(µl)          Water (µl)           Total (µl)

    0                  200                  200

    50                 150                  200

    100                100                  200

    150                50                   200
                                                                            Picture shows the method to determine total polysaccharide.
    200                0                    200                             Table shows glucose reference standard ( stock 100µg/ml)
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