Page 48 - 01 CARBOHYDRATES for TCM
P. 48
m-hydroxyphenyl method Method to determine total uronic
acid
Sulfuric acid
Sodium tetraborate
M-hydroxydiphenyl
Glucuronic acid
Ice
Microplate reader
96-well microplate
Glucoronic Water (µl) Total (µl)
acid(µl)
0 200 200
50 150 200
100 100 200
150 50 200
200 0 200 Figure show determine total uronic acid by
using m-hydroxydiphenyl method
Table show the amount of glucoronic acid
