Page 104 - MNU PM502 Ph. Micreo practical

Pharm D- Clinical Pharmacy Program Third Level Pharmaceutical Microbiology& Antimicrobials (PM 502)

LAB 10

Determination of antimicrobial susceptibility pattern

Antibiotic sensitivity test: an in vitro test of the sensitivity of bacteria to one or

more antibiotics by the diffusion technique on agar media.

• Its purpose is to guide the clinician in the choice of an antibiotic to treat a

bacterial infection and to use the data for monitoring bacterial resistance to

antibiotics.

Equipments

1- Plates of Muller Hinton agar

2- Antibiotic discs
3- Bacterial culture

4- Cotton swab
5- Forceps

Steps:
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1- An adjusted bacterial culture (1.0X10 CFU/ml) is disrupted on the surface

of agar plates using cotton swabs

➢ Immerse the swab in the suspension and remove excess liquid by

swirling the swab against the sides of the tube.

➢ Rub the entire surface of the agar dish three times, rotating the dish

approximately 60°c between streaks to ensure even distribution.

➢ Finally, run a swab around the edge of the agar to remove any excess

moisture.

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