Page 31 - MNU PM502 Ph. Micreo practical

Pharm D- Clinical Pharmacy Program Third Level Pharmaceutical Microbiology& Antimicrobials (PM 502)

Laminar Air Flow

• A laminar airflow chamber refers to an enclosed cabinet, which is equipped

with a HEPA (high-efficiency particulate air) filtered airflow system.

• The main purpose of a laminar flow cabinet/hood is to form a contaminant-free

work environment.

• it filters and captures all types of impurity particles entering the cabinet.

• It makes use of a filter pad and a special filter system known as a high-efficiency

particulate air filter or HEPA filter, which can remove the airborne impurity

particles that are up to 0.3 micrometres in size.

Uses

It provides an aseptic environment to perform different experiments.

It is generally used in microbiology laboratories in various procedures such as

media plate preparation, the culture of microorganisms, etc.,.

In the pharmaceutical industry, the preparation of drugs and medicines necessarily

requires a clean and sterile environment.

Parts of a Laminar Air Flow Chamber

1. Cabinet

Typically made up of stainless steel.

It provides insulation to the sterile environment created inside the laminar air flow

hood and protects it from the contamination and impure particles present outside.

2. Working Station

a solid base (stainless steel) to carry out processes such as plant tissue culture, the

culture of microorganisms, etc.

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