Page 79 - MNU PM502 Ph. Micreo practical

Pharm D- Clinical Pharmacy Program Third Level Pharmaceutical Microbiology& Antimicrobials (PM 502)

LAB 6

Determination of MIC by broth dilution method

MIC (Minimum Inhibitory Concentration): The lowest conc. of the antimicrobial

agent required to inhibit the growth of Microorganism.

Unit of MIC: μg/ml

MBC: Minimum Bactericidal Concentration.

The lowest conc. of the antimicrobial agent required to kill microorganism.

Importance:

Clinically, MIC is not only used to determine the amount of antibiotic that the

patient will receive but also the type of antibiotic used. This will lower the

opportunity for microbial resistance to specific antimicrobial agents.

Practical mission:

You are provided with certain antimicrobial agent (4 ml in volume) and required

to determine its MIC by broth dilution method.

Equipments:

1) 2 tubes of sterile nutrient broth.

2) 5 ml pipette.

3) 1 ml pipette.

4) 6 empty sterile test tubes.

5) Tube of antibiotic (Marked A).

6) Wassermann tube containing the culture.

7) Glass Pencil.

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