Page 88 - MNU PM502 Ph. Micreo practical
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Pharm D- Clinical Pharmacy Program Third Level Pharmaceutical Microbiology& Antimicrobials (PM 502)
LAB 7
Determination of MIC by agar diffusion method
Practical mission:
You are provided with certain antimicrobial agent (4 ml in volume) and required
to determine its MIC by agar diffusion method.
Equipments:
1) 3 empty sterile test tubes.
2) 2 sterile pipettes (5 ml and 1 ml).
3) 2 sterile Petri dishes.
4) Sterile Pasteur pipette.
5) Test tube containing sterile saline.
6) Test tube containing 4 ml of the initial conc. of the antibiotic (A).
7) Flask containing 50 ml of melted agar.
8) Wassermann tube containing the test M.O.
9) Loop.
Steps:
I- Preparation of Antibiotic Dilutions: (2-fold serial dilution of the test
antibiotic)
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