Page 169 - MNU-PM502- Pharmaeutical Microbiology Theoritical Book

Pharm D- Clinical Pharmacy Program Third Level Pharmaceutical Microbiology& Antimicrobials (PM 502)

• a volume (20–100 mL) of the antimicrobial agent at desired concentration is
introduced into the well.

• agar plates are incubated under suitable conditions depending upon the test

microorganism.

• The antimicrobial agent diffuses in the agar medium and inhibits the growth of
the microbial strain tested

Dilution methods

• Dilution methods are the most appropriate ones for the determination of MIC
values.

• variable amounts of antimicrobial substances incorporated into liquid or solid

media followed by inoculation of test bacteria

Agar dilution

Broth medium

➢ Macro-dilution

➢ Micro- dilution

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