Page 112 - MNU-PM503 Parasitology practical book

Pharm D- Clinical Pharmacy Program Third Level Parasitology and virology (PM501)

Example of viruses isolated by Cell Culture

Viruses readily isolated by cell Less frequently isolated viruses

culture

Herpes Simplex Varicella-Zoster

Cytomegalovirus Measles

Adenoviruses Rubella

Polioviruses Rhinoviruses

Coxsackie B viruses Coxsackie A viruses

Echoviruses

Influenza

Parainfluenza

Mumps

Respiratory Syncytial Virus

2- Serological assays:

• Detection of rising titers of antibody between acute and convalescent stages of

infection, or the detection of IgM in primary infection.

• The principles of serological assays are fundamentally the same, they depend

upon antibody-antigen interactions and consist of a known virus or viral

protein, a patient sample (usually serum), and an indicator. If antibodies are

present in the patient’s serum, they will bind to the virus. If no antibodies are

present, no binding will occur. The indicator is observed to determine whether

the sample is positive or negative for antibodies.

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