Page 121 - MNU-PM503 Parasitology practical book

Pharm D- Clinical Pharmacy Program Third Level Parasitology and virology (PM501)

• There are two variants:

1- Classical Immune electron microscopy (IEM) - the sample is treated with

specific anti-sera before being put up for EM. Viral particles present will be

agglutinated and thus congregate together by the antibody.

2- Solid phase immune electron microscopy (SPIEM) - the grid is coated with

specific anti-sera. Virus particles present in the sample will be absorbed onto

the grid by the antibody.

❑ Advantages and disadvantages of direct observation by Electron

Microscopy:

Advantages:

1- Rapid.

2- Detect viruses that cannot be grown in culture

3-Detects non-viable virus

Disadvantages:

1- Sensitivity often low

2- consume large number of samples.

3- limited to a few infections.

4- Expensive equipment & maintenance

5- Require experienced observer

D- Rapid Diagnosis Based on the Detection of Viral Antigens

- Nasopharyngeal Aspirate: RSV, Influenza A and B, Parainfluenza,

Adenovirus

- Faeces: Rotaviruses, Adenoviruses, Astrovirus

- Skin: HSV, VZV

108