Page 131 - MNUmicrobiology practical 2025

General Microbiology & Immunology (PM 401) second level Semester 4 2024/2025

ASO Test

A person infected with group A -hemolytic streptococci produces specific antibodies

against these exotoxins, one of which is antistreptolysin-O. The presence of antibodies

to streptolysin O indicates recent streptococcal infection in patients suspected with

acute rheumatic fever or post-streptococca glomerulonephritis following throat

infection. The quantity of this antibody in a patient's serum will establish the degree

of infection due to the group A -hemolytic streptococci.

Principle:

The ASO Latex test contains polystyrene latex particles, coated with purified and

stabilized Streptolysin-O (Antigen) which reacts with its corresponding

Antistreptolysin-O (Antibody) in the test sample resulting in the agglutination of latex

particles.

Intended use:

This diagnostic reagent kit is intended for the invitro determination of

Antistreptolysin - O activity in serum by slide agglutination method.

General Microbiology & Immunology (PM 401) Practical note Page 120