Page 22 - MNUmicrobiology practical 2025

General Microbiology & Immunology (PM 401) second level Semester 4 2024/2025

Resolution is the ability of the lenses to distinguish between two adjacent objects as
distinct and separate.

A compound light microscope has a maximum resolution of 0.2 µm,

this means it can distinguish between two points ≥ 0.2 µm, any objects closer than
0.2um will be seen as 1 object.

Microscope parts

1. Eyepiece/Ocular lens: Lens in which the final magnification occurs. Often it is

at 10X magnification but can be different.

2. Revolving nose piece: Holds multiple objective lenses in place. The base of the

nose piece can rotate, allowing each of the lenses to be rotated into alignment with
the ocular lens.

General Microbiology & Immunology (PM 401) Practical note Page 11