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Phylogenetic analysis of Omega-3 producing microalgae
isolated from mangrove area in Banda Aceh Indonesia

based on 18S rDNA gene.

1
2, 3
S H Anwar , T Rinanda and R Ramadhani 2
1 Department of Agricultural Product Technology, Universitas Syiah Kuala, Banda
Aceh, 23111, Indonesia
2 Department of Microbiology, Faculty of Medicine, Universitas Syiah Kuala, Banda
Aceh, 23111, Indonesia

E-mail: tristia.rinanda@unsyiah.ac.id

Abstract. The exploration and development of organisms producing Omega-3 have
escalated in order to fulfil the high demand for Omega-3 in every sector, especially in
human health. Microalgae are one of the highlighted microorganisms due to their
ability to produce edible oil or microbial oil containing Omega-3, such as
Thraustochytrium, Ulkenia and Schizochytrium. Besides Polyunsaturated Fatty Acids
(PUFA), the microbial oil from these microalgae also contains antioxidants and has
been developed as an alternative source of Omega-3. The objective of this study was to
identify the Omega-3 producing microalgae through genetic analysis. In this research
we conducted a phylogenetic analysis based of 18S rDNA gene of a microalga isolated
from mangrove leaves sourced from a mangrove area in Banda Aceh. The phylogenetic
analysis was conducted using Neighbor Joining method with bootstrap analysis. The
result revealed that the isolate was identified as a member of thraustochytrids family,
which was consistent to the morphological analysis of the isolate from our previous
research.

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