Page 6 - Practical Handout bio 2 mnu 2025_Neat

Level two – Semester 4 - PharmD Biochemistry-II PB-403 (Practical course) 2024/2025

3- Course Contents:

Lecture
Week Topics credit
No.
Hours
Carbohydrates: introduction to metabolism, digestion and
1. absorption of carbohydrates, Glycolysis and Regulation of 2
glycolysis.
2. Krebs's cycle 2

3. Glycogen metabolism. 2

4. HMP shunt and Uronic acid pathway 2
5. Monosaccharides interconversion. 2
6. Gluconeogenesis 2
7. Electron transport Chain & oxidative phosphorylation 2

8. Digestion and absorption of lipids. 2
9. Lipogenesis and lipolysis 2
10. Ketogenesis, ketolysis and phospholipids metabolism. 2
11. Protein metabolism, Protein digestion and absorption 2
12 General reactions of amino acids and urea cycle. 2
13 Individual amino acids metabolism (Glycine, Alanine, Serine 2

14 Individual amino acids metabolism (remaining amino acids) 2
Practical
Week Practical Topics credit
No.
hours
1. Biochemical testing & Infection Control Principles 1

2. Urine Analysis (Urine Composition and physical characters) 1
3. Urine Analysis (Pathological constituents in urine) 1
4. Urine report & microscopic examination 1
5. Principles of colorimetry and Automatic pipetting 1
6. Colorimetric assay of Glucose in urine and serum 1
Colorimetric assay of Liver Function Tests (serum proteins
7. 1
and enzymes)
8. Colorimetric assay of Liver Function Tests (Ammonia) 1
9. Colorimetric assay of Renal Function Tests (creatinine) 1
Colorimetric assay of Renal Function Tests (urea/BUN
10. levels) 1

11. Colorimetric assay of Renal Function Tests (uric acid levels) 1
12. Complete lipid profile 1
13. Cardiac Function tests 1

14. Practical Exam 1

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