Page 139 - 2014 Printable Abstract Book
P. 139
PS2 OMICS, EPIDEMIOLOGY, MUTAGENESIS, MITAGATORS, EPIGENETICS, MOUSE MODELS
(PS2-01) Genome wide analysis of chronic low-dose irradiated Drosophila melanogaster with
enhancement of locomotor behavior. Cha Soon KIM, PhD; In Kyung LEE, PhD; Ji Young KIM, PhD; Kwang
Hee YANG, PhD; and Seon Young NAM, PhD, Radiation Health Rearch Insititue, KHNP, Seoul, Korea,
Republic of
The study of radiation effects has been carrying out for decades. However, the biological effects
of low dose radiation (LDR) are still controversial. Thus, the biological effects of LDR should be investigated
to gain a more comprehensive understanding of the phenomenon and biological mechanism. The aim of
this study is to investigate the altered locomotor behavior and gene profiles of D. melanogaster upon LDR.
In this study, we measured locomotor behavior of D. melanogaster by larval pupation height and RING
(Rapid Iterative Negative Geotaxis) assay after exposure to 0.1 Gy (a dose rate of 16.7 mGy/hr) of γ-
irradiation. We also observed development processes (pupation and ecolosion rate) and longevity of fly
by chronic LDR. We performed whole genome expression analysis using gene expression microarray and
st
real-time PCR. As a result, pupation height was significantly increased after low dose irradiated 1 larval
instar. Moreover, the locomotion behavior of male flies was also significantly increased at 3~5 week after
low dose irradiation, but pupation and ecolosion rate and life span was not changed. To find relationship
with the alternation of locomotion behavior of flies by chronic LDR, we analyzed gene expression profiles.
Genome-wide analysis revealed that 344 probes were differentially expressed in irradiated larvae
compared with control. Among them, we found that several genes were categorized into larval behavior
related functional groups such as “locomotor behavior”, “oxidation reduction”. Also, genes involved in
conventional function groups, such as defense response, sensory perception, life span/aging were
included, and 5 candidate genes were confirmed using quantitative real-time PCR as differentially
expressed genes at irradiated larvae. Our data suggest that LDR evokes locomotion-related genes to
accelerate their motion throughout their lifetime, and these genes can be provides as potential markers
for LDR. [This work was financially supported from a research grant of MOTIE (No. 2013T100100018),
Republic of Korea.]
(PS2-02) Comparison of low- and high- doses of ionizing radiation using networks of co-regulated
1
2
biological processes. Yared H. Kidane and Francis Cucinotta, Universities Space Research Association,
2
1
Houston, TX and NASA, Lyndon B. Johnson Space Center, Houston, TX
Space travel, among other factors, expose human to Low Doses of Ionizing Radiation (LDIR). The
health impacts of LDIR are not well understood. Existing approaches that attempt to infer risks of LDIR
from High Dose Ionizing Radiation (HDIR) risk models have been controversial due to a nonlinear dose-
response relationship in LDIR range. Recent attempts involve use of host transcriptional profiles to study
commonalities and differences among host responses to LDIR and HDIR at molecular level. In this regard,
gene expression arrays provide a good platform in identification of LDIR- and HDIR- inducible genes and
biological processes. However, comparisons made at the level of genes, pathways, networks, and
biological functions have indicated that time-dependent responses are more apparent than dose-
dependent responses. This may in part be due to the transient nature of host responses which may result
in low level signal and concordance. Here, we propose an alternative approach for comparing host
137 | P a g e
(PS2-01) Genome wide analysis of chronic low-dose irradiated Drosophila melanogaster with
enhancement of locomotor behavior. Cha Soon KIM, PhD; In Kyung LEE, PhD; Ji Young KIM, PhD; Kwang
Hee YANG, PhD; and Seon Young NAM, PhD, Radiation Health Rearch Insititue, KHNP, Seoul, Korea,
Republic of
The study of radiation effects has been carrying out for decades. However, the biological effects
of low dose radiation (LDR) are still controversial. Thus, the biological effects of LDR should be investigated
to gain a more comprehensive understanding of the phenomenon and biological mechanism. The aim of
this study is to investigate the altered locomotor behavior and gene profiles of D. melanogaster upon LDR.
In this study, we measured locomotor behavior of D. melanogaster by larval pupation height and RING
(Rapid Iterative Negative Geotaxis) assay after exposure to 0.1 Gy (a dose rate of 16.7 mGy/hr) of γ-
irradiation. We also observed development processes (pupation and ecolosion rate) and longevity of fly
by chronic LDR. We performed whole genome expression analysis using gene expression microarray and
st
real-time PCR. As a result, pupation height was significantly increased after low dose irradiated 1 larval
instar. Moreover, the locomotion behavior of male flies was also significantly increased at 3~5 week after
low dose irradiation, but pupation and ecolosion rate and life span was not changed. To find relationship
with the alternation of locomotion behavior of flies by chronic LDR, we analyzed gene expression profiles.
Genome-wide analysis revealed that 344 probes were differentially expressed in irradiated larvae
compared with control. Among them, we found that several genes were categorized into larval behavior
related functional groups such as “locomotor behavior”, “oxidation reduction”. Also, genes involved in
conventional function groups, such as defense response, sensory perception, life span/aging were
included, and 5 candidate genes were confirmed using quantitative real-time PCR as differentially
expressed genes at irradiated larvae. Our data suggest that LDR evokes locomotion-related genes to
accelerate their motion throughout their lifetime, and these genes can be provides as potential markers
for LDR. [This work was financially supported from a research grant of MOTIE (No. 2013T100100018),
Republic of Korea.]
(PS2-02) Comparison of low- and high- doses of ionizing radiation using networks of co-regulated
1
2
biological processes. Yared H. Kidane and Francis Cucinotta, Universities Space Research Association,
2
1
Houston, TX and NASA, Lyndon B. Johnson Space Center, Houston, TX
Space travel, among other factors, expose human to Low Doses of Ionizing Radiation (LDIR). The
health impacts of LDIR are not well understood. Existing approaches that attempt to infer risks of LDIR
from High Dose Ionizing Radiation (HDIR) risk models have been controversial due to a nonlinear dose-
response relationship in LDIR range. Recent attempts involve use of host transcriptional profiles to study
commonalities and differences among host responses to LDIR and HDIR at molecular level. In this regard,
gene expression arrays provide a good platform in identification of LDIR- and HDIR- inducible genes and
biological processes. However, comparisons made at the level of genes, pathways, networks, and
biological functions have indicated that time-dependent responses are more apparent than dose-
dependent responses. This may in part be due to the transient nature of host responses which may result
in low level signal and concordance. Here, we propose an alternative approach for comparing host
137 | P a g e
