Page 144 - 2014 Printable Abstract Book
P. 144
information in the form of heatmaps, volcano plots, 3D visualization plots, correlation maps, and
metabolic pathway hit histograms. A urine metabolomics data set from a previously reported radiobiology
study in which samples were collected from mice exposed to gamma radiation was analyzed. MetaboLyzer
was able to identify 243 statistically significant ions out of a total of 1942. Numerous putative metabolites
and pathways were found to be relevant to radiation damage from the putative ion identification
workflow.
(PS2-11) Chemical approaches to elucidate the role of redox regulation in the gain of radiation
resistance in head and neck squamous cell carcinoma. Nelmi O. Devarie Baez, PhD; Jade Mims; Julie A.
Reisz, PhD; Allen W. Tsang, PhD; and Cristina M. Furdui, Wake Forest University School of Medicine,
Winston Salem, NC
Head and neck cancer (HNC) remains a non-discriminatory disease with more than fifty thousand
new cases diagnosed in the U.S. each year. Despite significant improvements with the use of combined
radiotherapy and targeted therapy, tumor recurrence and resistance to therapy are still major factors of
HNC mortality. To facilitate the development of new therapeutics for radiation resistant HNC, we
developed a matched model of radiation resistance for head and neck squamous cell carcinoma whose
biological features parallel clinical tissue specimens. Redox-status characterization of this cell-based
model revealed the upregulation of the antioxidant system in the radioresistant cells such as increased
levels of peroxiredoxins, reduced glutathione (GSH), and increased catalase to SOD ratio. Thus, one of our
research goals is to investigate how redox-dependent signaling controls the response to radiation
therapies. Proteins are known to carry regulatory function via the posttranslational modification of
cysteine residues caused by their interaction with reactive oxygen species (ROS) i.e sulfenic acids (Cys-
SOH) or sulfinic acids (Cys-SO2H), known as cellular “redox switches”. Due to the increased interest in
understanding how these subtle modifications can alter regulatory mechanisms on a wide number of
proteins, including those involved in the response to radiation therapies, the need for detection and
characterization of Cys-SOH/SO2H targets has triggered the development of chemical platforms to analyze
the redox-thiol proteome using imaging and mass spectrometry technologies. In this presentation, we
show our current efforts in the development of a high-throughput mass spectrometry-based method to
detect Cys-SOH and chemical approaches to detect Cys-SO2H by imaging and mass spectrometry. We
expect these methods will aid to elucidate functional roles of redox sensitive Cys and establish
mechanisms involved in the emergence of radiation resistance.
(PS2-13) Locoregional recurrence patterns in young versus elderly women treated for breast cancer.
2
1
1
1
Soumon Rudra ; David S. Yu, MD, Ph.D ; Esther S. Yu, MD ; Jeffrey M. Switchenko, PhD ; Donna Mister,
1
1
B.A. ; and Mylin A. Torres, MD, Emory University School of Medicine, Atlanta, GA and Tufts Medical
1
2
Center, Boston, MA
Objective: The purpose of this study was to examine locoregional recurrence patterns in breast
cancer patients who are not routinely screened by mammography due to age, women younger than 40
and those older than 75 years. Patients and Methods: The records of 230 breast cancer patients (n=125
less than age 40 and n=105 greater than age 75) who presented to the Emory University Department of
Radiation Oncology for curative treatment between 1997 and 2010 were reviewed. We compared disease
142 | P a g e
metabolic pathway hit histograms. A urine metabolomics data set from a previously reported radiobiology
study in which samples were collected from mice exposed to gamma radiation was analyzed. MetaboLyzer
was able to identify 243 statistically significant ions out of a total of 1942. Numerous putative metabolites
and pathways were found to be relevant to radiation damage from the putative ion identification
workflow.
(PS2-11) Chemical approaches to elucidate the role of redox regulation in the gain of radiation
resistance in head and neck squamous cell carcinoma. Nelmi O. Devarie Baez, PhD; Jade Mims; Julie A.
Reisz, PhD; Allen W. Tsang, PhD; and Cristina M. Furdui, Wake Forest University School of Medicine,
Winston Salem, NC
Head and neck cancer (HNC) remains a non-discriminatory disease with more than fifty thousand
new cases diagnosed in the U.S. each year. Despite significant improvements with the use of combined
radiotherapy and targeted therapy, tumor recurrence and resistance to therapy are still major factors of
HNC mortality. To facilitate the development of new therapeutics for radiation resistant HNC, we
developed a matched model of radiation resistance for head and neck squamous cell carcinoma whose
biological features parallel clinical tissue specimens. Redox-status characterization of this cell-based
model revealed the upregulation of the antioxidant system in the radioresistant cells such as increased
levels of peroxiredoxins, reduced glutathione (GSH), and increased catalase to SOD ratio. Thus, one of our
research goals is to investigate how redox-dependent signaling controls the response to radiation
therapies. Proteins are known to carry regulatory function via the posttranslational modification of
cysteine residues caused by their interaction with reactive oxygen species (ROS) i.e sulfenic acids (Cys-
SOH) or sulfinic acids (Cys-SO2H), known as cellular “redox switches”. Due to the increased interest in
understanding how these subtle modifications can alter regulatory mechanisms on a wide number of
proteins, including those involved in the response to radiation therapies, the need for detection and
characterization of Cys-SOH/SO2H targets has triggered the development of chemical platforms to analyze
the redox-thiol proteome using imaging and mass spectrometry technologies. In this presentation, we
show our current efforts in the development of a high-throughput mass spectrometry-based method to
detect Cys-SOH and chemical approaches to detect Cys-SO2H by imaging and mass spectrometry. We
expect these methods will aid to elucidate functional roles of redox sensitive Cys and establish
mechanisms involved in the emergence of radiation resistance.
(PS2-13) Locoregional recurrence patterns in young versus elderly women treated for breast cancer.
2
1
1
1
Soumon Rudra ; David S. Yu, MD, Ph.D ; Esther S. Yu, MD ; Jeffrey M. Switchenko, PhD ; Donna Mister,
1
1
B.A. ; and Mylin A. Torres, MD, Emory University School of Medicine, Atlanta, GA and Tufts Medical
1
2
Center, Boston, MA
Objective: The purpose of this study was to examine locoregional recurrence patterns in breast
cancer patients who are not routinely screened by mammography due to age, women younger than 40
and those older than 75 years. Patients and Methods: The records of 230 breast cancer patients (n=125
less than age 40 and n=105 greater than age 75) who presented to the Emory University Department of
Radiation Oncology for curative treatment between 1997 and 2010 were reviewed. We compared disease
142 | P a g e
