Page 380 - 2014 Printable Abstract Book
P. 380
system under basal conditions but also prevents the cell from adapting to an oxidative environment. The
synergistic effects of increased ROS, accumulated DNA damage and impaired DNA repair could participate
and partly explain, the cancer-prone phenotype in Gorlin’s syndrome. To our knowledge, this is the first
study showing the impact of PTCH1 mutations on the oxidative DNA repair capacities.
(PS7-47) Combinations of aromatic and aliphatic hydrocarbon radiolysis. Jay A. LaVerne, Universtiy of
Notre Dame, Notre Dame, IN
Aliphatic and aromatic hydrocarbons have very different sensitivities to gamma radiolysis and
aromatic compounds are generally considered to be radiation inert. A wide range of outcomes can be
obtained when various combinations of these compounds are irradiated. This work will examine the
decomposition of aromatic compounds especially in combination with aliphatic substituents or in
mixtures. The purpose is to determine the contribution of an aliphatic entity or an aromatic one in the
overall decomposition of the system. Aromatic compounds with different length aliphatic side chains are
compared to each other and to the results of mixtures of aromatic and aliphatic compounds. Simple
organic radiolysis mechanisms will be discussed and applied to the more complicated systems containing
combinations of aliphatic and aromatic compounds.
(PS7-48) The effects of space radiation to mouse embryonic stem cells in international space station.
Takashi Morita, Osaka City University, Osaka, Japan
It is important to evaluate the influence of space radiation on human body or mammalian cells
during a longer stay in space including missions to International Space Station (ISS), the moon of the earth,
or Mars. Mouse embryonic stem (ES) cells are good candidate to estimate the space radiation effects,
because they have normal karyotypes of chromosomes. We prepared moue ES cells including wild-type,
GFP-bearing cells, histone H2AX-deficient cells. The five packages, each containing100 frozen cell tubes
were made. They were launched by Space X-II in March 2013 as project named “Stem Cells”. They have
been kept in the Japanese experiment module called “KIBO” in ISS and stored in a freezer (MELFI) at -95C.
The ES cells exposed to space radiation will be examined DNA damages by detecting histone H2AX foci
and their chromosomal aberrations by FISH. The ES cells can be also microinjected into normal embryos
and cultured in vitro to see the developmental defects. Now (after 1 year and about 2 months of the
launch), one of the packages has come back to the ground and we are investigating exposed dose of the
ES cell package and maintenance of the temperature. The ES cells were thawed and their viabilities were
tested. We are now underway of the investigation of the effect of space radiation.
(PS7-49) Mutations identified in cell clones derived from X-irradiated human cultured cells by whole
2
1
3
genome sequencing. Yasunari Satoh ; Akihiro Fujimoto ; and Jun-ichi Asakawa , Department of Genetics,
1
Radiation Effects Research Foundation, Hiroshima, Japan ; Center for Integrative Medical Sciences, RIKEN,
Yokohama, Japan ; and Departmet of Genetics, Radiation Effects Research Foundation, Hiroshima, Japan
3
2
To comprehensively analyze radiation-induced mutation, we conducted whole genome
sequencing of single cell-derived clones established from an X-irradiated EB-virus transformed human B
378 | P a g e
synergistic effects of increased ROS, accumulated DNA damage and impaired DNA repair could participate
and partly explain, the cancer-prone phenotype in Gorlin’s syndrome. To our knowledge, this is the first
study showing the impact of PTCH1 mutations on the oxidative DNA repair capacities.
(PS7-47) Combinations of aromatic and aliphatic hydrocarbon radiolysis. Jay A. LaVerne, Universtiy of
Notre Dame, Notre Dame, IN
Aliphatic and aromatic hydrocarbons have very different sensitivities to gamma radiolysis and
aromatic compounds are generally considered to be radiation inert. A wide range of outcomes can be
obtained when various combinations of these compounds are irradiated. This work will examine the
decomposition of aromatic compounds especially in combination with aliphatic substituents or in
mixtures. The purpose is to determine the contribution of an aliphatic entity or an aromatic one in the
overall decomposition of the system. Aromatic compounds with different length aliphatic side chains are
compared to each other and to the results of mixtures of aromatic and aliphatic compounds. Simple
organic radiolysis mechanisms will be discussed and applied to the more complicated systems containing
combinations of aliphatic and aromatic compounds.
(PS7-48) The effects of space radiation to mouse embryonic stem cells in international space station.
Takashi Morita, Osaka City University, Osaka, Japan
It is important to evaluate the influence of space radiation on human body or mammalian cells
during a longer stay in space including missions to International Space Station (ISS), the moon of the earth,
or Mars. Mouse embryonic stem (ES) cells are good candidate to estimate the space radiation effects,
because they have normal karyotypes of chromosomes. We prepared moue ES cells including wild-type,
GFP-bearing cells, histone H2AX-deficient cells. The five packages, each containing100 frozen cell tubes
were made. They were launched by Space X-II in March 2013 as project named “Stem Cells”. They have
been kept in the Japanese experiment module called “KIBO” in ISS and stored in a freezer (MELFI) at -95C.
The ES cells exposed to space radiation will be examined DNA damages by detecting histone H2AX foci
and their chromosomal aberrations by FISH. The ES cells can be also microinjected into normal embryos
and cultured in vitro to see the developmental defects. Now (after 1 year and about 2 months of the
launch), one of the packages has come back to the ground and we are investigating exposed dose of the
ES cell package and maintenance of the temperature. The ES cells were thawed and their viabilities were
tested. We are now underway of the investigation of the effect of space radiation.
(PS7-49) Mutations identified in cell clones derived from X-irradiated human cultured cells by whole
2
1
3
genome sequencing. Yasunari Satoh ; Akihiro Fujimoto ; and Jun-ichi Asakawa , Department of Genetics,
1
Radiation Effects Research Foundation, Hiroshima, Japan ; Center for Integrative Medical Sciences, RIKEN,
Yokohama, Japan ; and Departmet of Genetics, Radiation Effects Research Foundation, Hiroshima, Japan
3
2
To comprehensively analyze radiation-induced mutation, we conducted whole genome
sequencing of single cell-derived clones established from an X-irradiated EB-virus transformed human B
378 | P a g e
