Page 382 - 2014 Printable Abstract Book
P. 382
is achieved only within 24 hours. More than 80% of γH2AX/53BP1 foci caused by the action of 11B ions
form clusters, consisting of several individual foci, which repair is significantly slower compared to the γ-
induced foci. Our results further show that clusters are formed along the track of passing 11B particles in
the first minutes after irradiation. During the PI period the increasing of the size of clusters which consist
of 2-17 individuals closely spaced γH2AX/53BP1 foci was observed. We assume that this change could be
associated with the accumulation of protein markers in DSBs repair sites as well as with the gradual
decondensation of chromatin in the place of DSBs and with the enzymatic formation of DSBs.
(PS7-51) Development of Argon-Based Laser Produced Plasma Source of Soft X-rays for Radiobiology
1
1
1
1
2
Studies. Daniel Adjei ; Mesfin Getachew. Ayele ; Przemyslaw Wachulak ; Andrzej Bartnik ; Ludek Vysin ;
3
3
1
3
1
Henryk Fiedorowicz ; Lukasz Wegrzynski ; Anna Wiechecka ; Janusz Lekki ; and Wojciech M. Kwiatek
1
Instittute of Optoelectronics, Warsaw, Poland ; Institute of Physics, Czech Technical University, Prague,
Czech Republic ; and Institute of Nuclear Physics PAN, Krakow, Poland
2
3
Abstract- A desk-top laser produced plasma source of soft X-ray has been developed for
radiobiology research. The source is based on a double-stream gas puff target, irradiated with a
commercial Nd: YAG laser (EKSPLA), which generates laser pulses of 4 ns time duration and energy of ~740
mJ at 10 Hz repetition rate. The source has been optimized for maximum emission in the X-ray “water
window” spectral wavelength range from 2.3 nm to 4.4 nm wavelength (540 eV to 281 eV) by using argon
gas puff target and spectral filtering. Results of the source characterization measurements and dosimetry
of the produced soft X-ray radiation will be discussed. The source emits high intensity pulses of soft X-
rays, which are useful to understand the mechanisms of high dose effects of low energy X-rays on
biological samples. The high brightness of the laser produced plasma soft X-ray source and the low
penetration depth of the produced X-ray radiation in biological specimen allow a high photon fluence of
6
2
over (5.1±0.5)x10 photons/µm /shot to be delivered to the specimen. The source has a unique capability
for irradiation of specimen with high pulse photon flux in vacuum and in the near future in He-
environment. The source has been demonstrated to induce both single- and double-strand breaks in
pBR322 plasmid DNA. When the irradiated sample was analysed by an agarose gel electrophoresis
method, we observed an increase in the efficiency of induction of strand breaks in supercoiled DNA as a
function of photon flux.
(PS7-52) Protection and immune response induced in Toxoplasma gondii mouse models by 60Co
gamma-irradiated extracts of tachyzoites without adjuvants. PrimaryAuthor.AuthorBlock:
2
3
1
1
Andrea da Costa ; Nahiara E. Zorgi, MSc. ; Nanci do Nascimento, MS., PhD ; Andres J.G. Júnior, MSc, PhD ;
1
1
and Heitor F.A. Júnior, MSc., PhD, PHD, Instituto de Medicina Tropical de São Paulo, São Paulo, Brazil ;
2
Instituto de Ciências Biomédicas da Universidade de São Paulo, São Paulo, Brazil ; and Instituto de
3
Pesquisas Energéticas e Nucleares, São Paulo, Brazil
We evaluated the effect of ionizing radiation on soluble extracts of tachyzoites of T. gondii (AgTg)
and the ability of these extracts to induce immunity in BALB/c mice. A tachyzoite extract from the RH
60
strain was purified and CO irradiated at 500, 1000, 1500, 2000 and 4000 Gy. A dose response effect of
the radiation was induced on extract as observed by SDS-PAGE, but antigenicity was maintained at lower
380 | P a g e
form clusters, consisting of several individual foci, which repair is significantly slower compared to the γ-
induced foci. Our results further show that clusters are formed along the track of passing 11B particles in
the first minutes after irradiation. During the PI period the increasing of the size of clusters which consist
of 2-17 individuals closely spaced γH2AX/53BP1 foci was observed. We assume that this change could be
associated with the accumulation of protein markers in DSBs repair sites as well as with the gradual
decondensation of chromatin in the place of DSBs and with the enzymatic formation of DSBs.
(PS7-51) Development of Argon-Based Laser Produced Plasma Source of Soft X-rays for Radiobiology
1
1
1
1
2
Studies. Daniel Adjei ; Mesfin Getachew. Ayele ; Przemyslaw Wachulak ; Andrzej Bartnik ; Ludek Vysin ;
3
3
1
3
1
Henryk Fiedorowicz ; Lukasz Wegrzynski ; Anna Wiechecka ; Janusz Lekki ; and Wojciech M. Kwiatek
1
Instittute of Optoelectronics, Warsaw, Poland ; Institute of Physics, Czech Technical University, Prague,
Czech Republic ; and Institute of Nuclear Physics PAN, Krakow, Poland
2
3
Abstract- A desk-top laser produced plasma source of soft X-ray has been developed for
radiobiology research. The source is based on a double-stream gas puff target, irradiated with a
commercial Nd: YAG laser (EKSPLA), which generates laser pulses of 4 ns time duration and energy of ~740
mJ at 10 Hz repetition rate. The source has been optimized for maximum emission in the X-ray “water
window” spectral wavelength range from 2.3 nm to 4.4 nm wavelength (540 eV to 281 eV) by using argon
gas puff target and spectral filtering. Results of the source characterization measurements and dosimetry
of the produced soft X-ray radiation will be discussed. The source emits high intensity pulses of soft X-
rays, which are useful to understand the mechanisms of high dose effects of low energy X-rays on
biological samples. The high brightness of the laser produced plasma soft X-ray source and the low
penetration depth of the produced X-ray radiation in biological specimen allow a high photon fluence of
6
2
over (5.1±0.5)x10 photons/µm /shot to be delivered to the specimen. The source has a unique capability
for irradiation of specimen with high pulse photon flux in vacuum and in the near future in He-
environment. The source has been demonstrated to induce both single- and double-strand breaks in
pBR322 plasmid DNA. When the irradiated sample was analysed by an agarose gel electrophoresis
method, we observed an increase in the efficiency of induction of strand breaks in supercoiled DNA as a
function of photon flux.
(PS7-52) Protection and immune response induced in Toxoplasma gondii mouse models by 60Co
gamma-irradiated extracts of tachyzoites without adjuvants. PrimaryAuthor.AuthorBlock:
2
3
1
1
Andrea da Costa ; Nahiara E. Zorgi, MSc. ; Nanci do Nascimento, MS., PhD ; Andres J.G. Júnior, MSc, PhD ;
1
1
and Heitor F.A. Júnior, MSc., PhD, PHD, Instituto de Medicina Tropical de São Paulo, São Paulo, Brazil ;
2
Instituto de Ciências Biomédicas da Universidade de São Paulo, São Paulo, Brazil ; and Instituto de
3
Pesquisas Energéticas e Nucleares, São Paulo, Brazil
We evaluated the effect of ionizing radiation on soluble extracts of tachyzoites of T. gondii (AgTg)
and the ability of these extracts to induce immunity in BALB/c mice. A tachyzoite extract from the RH
60
strain was purified and CO irradiated at 500, 1000, 1500, 2000 and 4000 Gy. A dose response effect of
the radiation was induced on extract as observed by SDS-PAGE, but antigenicity was maintained at lower
380 | P a g e
