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1st Int. Transborder Conf. of the Timor Island: Timor %u2013 Science without borderDili, 7-8 May 202549Supplementation of Cellulase Enzyme in a Complete Feed Based on Fermented Banana Stem On Nutritional Digestibility of Fattened Bali CattleY.U. L. Sobang*1, C. A. Paulus1, S. P. Nawa Pau2and F. D. Samba11Fac. Anim. Husb, Marine and Fishery Sciences, Universitas Nusa Cendana, Indonesia2Faculty of Economics and Business, Universitas Nusa Cendana, Indonesia*Corresponding author: yulsobang@staf.undana.ac.idAbstractOne of the main constraints to increasing beef cattle production in East Nusa Tenggara, particularly on Timor Island, is the insufficient feed quality provided by farmers, which often fails to meet the dry matter requirements for beef cattle. This highlights the need for effective supplementation strategies. The addition of exogenous cellulase enzymes offers a potential solution to improve feed digestibility in the rumen, particularly in areas with extended dry seasons where available feed consists largely of agricultural and plantation waste. The objective of this study was to evaluate the effectiveness of exogenous cellulase enzyme supplementation at different inclusion levels. The study used nine male Bali cattle aged 1 to 1.5 years, with body weights ranging from 108 to 116 kg (average 112.79 kg; CV = 5.72%). A Completely Randomized Design was applied with three treatments and three replications. The treatments were as follows: P0= 70% Lamtoro green fodder + 30% complete feed (control), P1 = 70% Lamtoro green fodder + 30% complete feed + 5 g cellulase enzyme/kg dry matter of complete feed, P2= 70% Lamtoro green fodder + 30% complete feed + 10 g cellulase enzyme/kg dry matter of complete feed. The results showed that dry matter digestibility ranged from 72.4% to 74.0%, organic matter digestibility ranged from 71.48% to 75.22%, and crude protein digestibility ranged from 65.05% to 69.58%. Cellulase enzyme supplementation significantly improved the digestibility of dry matter and organic matter but had no significant effect on crude protein digestibility. It is concluded that cellulase enzyme supplementation in complete feed based on fermented banana stem can enhance the digestibility of dry matter and organic matter in fattened Bali cattle, although it does not significantly affect crude protein digestibility across different enzyme levels.Keywords: banana stem, cellulase, fermentation, complete feed, Bali cattle.