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1st Int. Transborder Conf. of the Timor Island: Timor %u2013 Science without borderDili, 7-8 May 202577The Effect of Chromolaena odorata Biofermentation Duration Using Palm pith Flour As A Carbon Source On Fiber, Calcium, And Phosphorus Content of the Fermented ProductErches Pellondou, Twen O. Dami Dato and Marthen L. Mullik*Fakultas Peternakan, Kelautan dan Perikanan, Universitas Nusa CendanaJl. Adi Sucipto, Penfui, Kupang, Nusa Tenggara Timur, 85001*Corresponding author:marthenmullik@staf.undana.ac.idAbstractThis study aimed at evaluating the effect of biofermentation duration of Chromolaena odorata using palm pith flour as a carbon source on the fiber, calcium, and phosphorus content of the fermented product.The experimental design used was a Completely Randomized Design (CRD) to test 4 treatments, each replicated 4 times. The treatments were: fermentation duration of C. odorata for 21 days (LB-21), 14 days (LB-14), 7 days (LB-7), or no fermentation (LB-0). The observed variables included fiber, calcium, and phosphorus content. The data obtained were analyzed using analysis of variance (ANOVA) to determine the effect of the treatments, and Duncan's Multiple Range Test was used to detect differences between treatments. The results showed that biofermentation of C. odorata significantly reduced the NDF (Neutral Detergent Fiber) content in a linear pattern, from 79.19% in the non-fermented C. odorata (LB-0) to 70.44% with the longest fermentation time (21 days; LB-21). Similarly, ADF (Acid Detergent Fiber) content significantly decreased from 45.21% in LB-0 to 32.09% in LB21. Conversely, calcium and phosphorus content increased significantly. Calcium increased from 0.64% (LB-0) to 0.87% (LB-21), while phosphorus content increased from 0.41% (LB-0) to 0.52% in LB-21. It can be concluded that longer biofermentation of C. odorata decreases NDF and ADF content, but increases the mineral content of calcium and phosphorus in the product, with the optimal fermentation duration being 21 days.Keywords: biofermentation, Chromolaena odorata, rumen fluid, palm pith.