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EFFICACY

COMPARISON OF CYTOTOXIC EFFECTS ON RABBIT

CORNEAL ENDOTHELIUM BETWEEN PRESERVATIVE-FREE

AND PRESERVATIVE-CONTAINING DORZOLAMIDE/ TIMOLOL

Kwon J, et al. Korean J Ophthalmol 2015;29(5):344-350.

ABSTRACT

PURPOSE: To evaluate and compare the toxic effects of eyedrops containing a fixed combination of
2.0% dorzolamide and 0.5% maleate timolol with or without preservatives on rabbit corneal endo-
thelium.

METHODS: In vivo study on 11 rabbits comparing Dorzolamide/Timolol eyedrops with preservative
or without preservative. The left eye was treated with BAK DTFC while the right eye was treated with
preservative free DTFC: eyedrops were injected into the anterior chamber. Corneal endothelium toxic
damages are evaluated after 24 hours.

STUDY CRITERIA:
X Clinical exam: corneal haze and conjunctival and limbal vascular injection were
graded from 0 (none) to 4 (severe).

X TUNEL: detection of apoptotic cells (cell death).

RESULTS:
X Slit lamp photograph shows 24 hours after injection of BAK DTFC and PF DTFC :
X A: BAK DTFC. The eye show severe corneal haze (2.8± 0.6) and conjunctival vascular
injection (2.4± 0.8)

X B: PF DTFC. The eye show minimal corneal haze (0.4± 0.5) and conjunctival vascular
injection (0.6±0.5)

Figure 8: 24 hours after injection Figure 9: 24 hours after injection
of BAK DTFC of PF DTFC

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