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                 The median number of dead cells from 5 consecutive microscopic fields on each eye was 28 in the BAK
                 DTFC group and 2 in the PF DTFC group (p < 0.001).


                 The median number of dead cells from 5 consecutive microscopic fields on each eye was 28 in the BAK
                 DTFC group and 2 in the PF DTFC group (p < 0.001).
                 The median number of TUNEL-positive endothelial cells, which were counted from 5 consecutive microsco-
                 pic fields (×400), was 4 in the BAK DTFC group and 0 in the PF DTFC group (p < 0.001).


























                                             Figure 10: Evaluation of corneal endothelium toxic damages

                 CONCLUSIONS: Moreover, BAK DTFC treated eyes exhibited prominent apoptosis of endothelial cells. The
                 main cause of endothelial toxicity is the preservative.








































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