Page 130 - Mesenchymal Stem cells, Exosomes and vitamins in the fight aginst COVID

patient’s families of a critical condition.

However, the patient felt better on February 3, for instance, the shortness of breath was significantly
recovering. On February 4, the C-reactive protein decreased to 13.6 g/L, and the absolute
lymphocyte count rose to 0.58 × 10 /L, which indicated that the patient was recovering rapidly. The
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indexes of liver and myocardium function recovered. Both fever and shortness of breath disappeared
on February 5. He was rolled out of ICU. On February 9, the fourth chest CT confirmed that the

pneumonia was disappearing. On February 13, the C-reactive protein concentration was 10.1 g/L,
and the absolute lymphocyte count was 0.93 × 10 /L. Up to now, the patient felt much better.
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Supplementary 4: More results of the 10 x RNA-seq surevey

Flow cytometry analysis
The PI staining results showed that 91.60% of the total cell population was alive, and the cells were:
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CD105 , CD90 , CD73 , CD44 , CD29 , CD14 and CD45 (Supplemental Figure 1).
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chinaXiv:202002.00080v1

Supplemental Figure 1. Flow cytometry evaluation of transplanted MSCs (A) Single cells (87%)
were gated firstly. (B) Live cells (91% of the single cells) were enrolled. (C-F) 99% of selected cells
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were CD105 , CD90 , CD73 , CD44 , CD29 , CD14 and CD45 .
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The overview of the survey
A deep transcriptional states map of MSCs and gene expression at single-cell level was generated
after the performance of 10× Genomics high throughput of RNA sequencing. The 12,500 cells were
acquired in the survey, leading to 881,215,280 raw reads totally. The median number of genes and
UMIs detected per cell were 4,099 and 23,971, respectively (Supplemental Figure 2). The
sequencing saturation rate was 72.9%, which met the scRNA-seq requirements.

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