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1436 uMSC Exosomes Suppress Scar Formation
Figure 6. Inhibition of uMSC-Exo-specific miRNAs abolished the ability of uMSC-Exos to suppress TGF-b/SMAD2 activation in vitro. (A): Sche-
matic showing the procedure of preparing Antago-uMSC-Exos. (B): Quantitative reverse transcription-polymerase chain reaction (qRT-PCR)
showing the miRNA level of fibroblasts treated with modified uMSC-Exos. The inhibitory efficiency of Antago-uMSC-Exos is shown compared
with NC-uMSC-Exos. Equal amounts of phosphate-buffered saline (PBS) were used as exosome control (Mock). Data are presented as mean 6 SD;
n =3; pp, p , .01. (C): The effect of modified exosomes on SMA expression level assessed by both Western blot (upper) and qRT-PCR (lower).
EqualamountsofPBSservedas negativecontrol. Dataarepresentedas mean6SD;n=3;pp,p,.01.(D):Flowcytometry analysisshowedthe
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