Page 82 - Ebook-Book JCMS 2025

Cannabis Oil Modulates Gut Microbiota and Short Chain Fatty
Acids Pathways in Breast Cancer
May Soe Thu1, Thunnicha Ondee2, Sunchai Payungporn3, Kamonwan Banchuen4, Mawin Vongsaisuwon5
,
Chanida Vinayanuwattikun6, Phanupong Phutrakool7, Preecha Nootim4, Barry J. Campbell8, Joanne L.
Fothergill9, Nattiya Hirankarn1, Krit Pongpirul2*
1 Center of Excellence in Immunology and Immune-Mediated Diseases, Department of Microbiology,
Faculty of Medicine, Chulalongkorn University
2 Center of Excellence in Preventive and Integrative Medicine, Department of Preventive and Social
Medicine, Faculty of Medicine, Chulalongkorn University
3 Center of Excellence in Systems Microbiology, Department of Biochemistry, Faculty of Medicine,
Chulalongkorn University
4 Department of Thai Traditional and Alternative Medicine, Ministry of Public Health
5 Department of Surgery, Faculty of Medicine, Chulalongkorn University
6 Department of Medicine, Faculty of Medicine, Chulalongkorn University
7 Research Affairs, Faculty of Medicine, Chulalongkorn University
8 Department of Infection Biology & Microbiomes, University of Liverpool
9 Department of Clinical Infection, Microbiology and Immunology, Institute of Infection, Veterinary &
Ecological Sciences, University of Liverpool
*Corresponding Author E-mail: doctorkrit@gmail.com
Background: Methods: Abstract
The gut microbiome and short-chain fatty acids (SCFAs) significantly influence immunity,
inflammation, and cancer metabolism. Chemotherapy disrupts microbial communities
and alters SCFA production. Cannabinoids have immunomodulatory potential, but their
effects on gut microbiota and SCFA pathways in cancer remain unclear. Therefore, we aim to
elucidate the effects of cannabis oil on fecal SCFAs and gut microbiota in breast cancer
patients undergoing chemotherapy.
In a randomized, double-blind, placebo-controlled trial (TCTR20220809001, CBC2), 10
breast cancer patients received cannabis oil (n=6) or placebo (n=4) daily for 12 weeks.
Paired fecal samples at baseline and week 12 were analyzed for SCFAs via HPLC and
microbiota via 16S rRNA sequencing.
80 Joint Conference in Medical Sciences 2025