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Immunomodulatory Effects of Andrographis paniculata
Extracts on Wound Healing
Ladawan Khowawisetsut1,2, Narinee Srimark2, Saracha Pattanapholkornsakul3, Kanyanut Insawang4,5
,
Payalak sudcharee2, Nantaporn Namviriyachote6, Patimaporn Wongprompitak7, Primana Punnakitikashem4,5
,
Pravit Akarasereenont8, Pronprom Muangman6*
1 Department of Parasitology, Faculty of Medicine, Siriraj Hospital, Mahidol University
2 Siriraj Center of Research Excellence for Microparticle and Exosome in Diseases, Faculty of Medicine,
Siriraj Hospital, Mahidol University
3 Center of Applied Thai Traditional Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University
4 Siriraj Center of Research Excellence in Theranostic Nanomedicine, Faculty of Medicine, Siriraj Hospital,
Mahidol University
5 Department of Biochemistry, Faculty of Medicine, Siriraj Hospital, Mahidol University
6 Department of Surgery, Faculty of Medicine, Siriraj Hospital, Mahidol University
7 Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University
8 Department of Pharmacology, Faculty of Medicine, Siriraj Hospital, Mahidol University
Background: *Corresponding Author E-mail: pornprom.mua@mahidol.edu
Abstract
Andrographolide (ADG), the bioactive compound in Andrographis paniculata, possesses
anti-inflammatory, antioxidant, antimicrobial, and immunomodulatory properties relevant
to wound healing. Neutrophils and macrophages play roles during the inflammatory
phase, with neutrophils facilitating pathogen clearance and macrophages modulating
fibroblast activity through cytokine production. The regulation of neutrophil apoptosis and
macrophage function is critical for effective resolution of inflammation. This study aimed to
investigate the effects of institutionally prepared A. paniculata extracts (APe) on neutrophil
and macrophage functions implicated in the wound healing process.
Methods: APe was prepared by the Center of Applied Thai Traditional Medicine, Faculty of Medicine
Siriraj Hospital, Mahidol University. The cytotoxicity of APe on THP-1 cells was assessed
using the MTS assay to determine the optimal concentration for subsequent experiments.
APe-induced changes in neutrophil apoptosis and ROS production were measured by
flow cytometry. Additionally, the influence of APe on macrophage phenotype and cytokine
induction in THP-1-derived macrophages was analyzed through gene expression profiling
using RT-PCR.
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