Page 25 - Practical-workbook-organic-3 isa
P. 25
Procedure of Osazone Synthesis
In a 250 ml stoppered flask:
◼ Dissolve 0.5 gm of glucose in 5 ml water in the flask.
◼ Add 2 gm sod acetate with shaking, then add 2 ml acetic acid.
◼ Add 1 ml of phenylhydrazine.
◼ Reflux using a condenser and water bath for 30 min.
◼ Cool the reaction mixture at room temperature.
◼ To purify the product:
1. Add 50 ml of water to eliminate any byproduct.
2. Then filter to get a yellow ppt of osazone.
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