Page 100 - ebook HCC

EASL HCC SUMMITHCC SUMMIT
PROGRAMME
GENEVA, SWITZERLANDA, SWITZERLAND
99
98
98 PROGRAMME AND ABSTRACTSAND ABSTRACTS GENEV EASL 99
FEBRUAR
FEBRUARY 13 - 16, 2014Y 13 - 16, 2014
Poster Board Number B6
VARIATION IN HEPATITIS B VIRUS GENOME IN
PATIENTS OF HEPATOCELLULAR CARCINOMA
FROM NORTHERN, SOUTHERN AND NORTH
EAST INDIA

1
2
Manash P. Sarma , Giasuddin Ahmed , Subhash Medhi , Premashis Kar 1 Table 1 (on page 88): Whole genome analysis of HBV genome in three different
1
1 Medicine, Maulana Azad Medical College, New Delhi, geographical locations from HCC and asymptomatic HBV carriers (significant
2 Biotechnology, Gauhati University, Guwa, India mutations are tabulated).
Corresponding author’s e-mail: manash3268@gmail.com [Salient feature of the Table : Novel mutations documented from the HCC cases in the
present study are tabulated. The are as follows: surface (132 stop), polymerase (frameshift
at codon 178), core (10 IàL, 41 SàT, 92 VàG, 96 NàT and 164 QàP) and X (33 PàS)
Introduction: Mutation throughout HBV genome is significantly associated with HCC and gene. These mutations have been reported for the first time and were found significantly
varies with respect to its geographical location. associated in HCC cases from the three regions]
(NI: North India, SI: South India, NEI: North East India, * ND: Not detected, aa: Amino acid
Aims: Study was aimed to analyse the whole genome of HBV in HCC cases from three and NS: Not studied)
regions of India.
BASIC POSTER ABSTRACTS sets of walking primers, amplicons were sequenced, submitted to http://www.ncbi.nlm.nih. BASIC POSTER ABSTRACTS
Methodology: 75 HBV related HCC cases were included. HBV DNA was amplified by six
gov, translated into amino acid and aligned using BioEdit v7.0.9.

Results: 60, 15, 23 and 1 mutations were observed in PC/C, X, P and S genes respectively.
Mutations like 10IàL was significantly associated in HCC cases from NEI [(OR=5. 63) VS
SI] & [(p <0.01; OR=16.63) VS NI]. Mutations like 41SàT (p< 0.001; OR= 19.01), 92 VàG
(p< 0.001; OR= 19.01), 96NàT (p< 0.001; OR= 19.01) and 164QàP (OR= 3.085) were
significantly associated with HCC cases from NI. Widely reported 28 Wàstop mutation
was found in few HCC cases. 132àstop [(p= 0.004; OR= 5.479 VS SI) & [(p= 0.004; OR=
5.479) VS NEI] was interesting. 267IàN and 268DàT were exclusively to HCC from NEI
while 270S→F with NI. Reported drug mutants (80LàI, 236NàT, 169I→T and 181A→V)
were observed.

Conclusions: PC/C was most prone to mutation followed by P, X and S. Maximum
variation in HBV genome was observed in HCC cases from NI followed by NEI and SI.
Noval mutations in surface (132 stop), polymerase (178 frameshift), core (10 IàL, 41 SàT,
92 VàG, 96 NàT, 164 QàP) and X (33 PàS) gene needs advanced research.