Page 110 - ebook HCC

GENEV
PROGRAMME
EASL HCC SUMMITHCC SUMMIT
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108 PROGRAMME AND ABSTRACTSAND ABSTRACTS GENEVA, SWITZERLANDA, SWITZERLAND EASL 109
FEBRUAR
FEBRUARY 13 - 16, 2014Y 13 - 16, 2014
Poster Board Number B11
HUMAN AMNIOTIC MEMBRANE-DERIVED
PROTEINS DISPLAY ANTICANCER ACTIVITY IN
HEPATOCELLULAR CARCINOMA

Ana C. Mamede 1 2 3 , Sara Guerra , Mafalda Laranjo , Ana F. Brito ,
1 4
1 3
1 3
Kathleen Santos , Maria J. Carvalho 1 3 5 , José G. Tralhão , Paulo Moura , Results: After treatment with hAMPE, protein synthesis decreased 85% in Hep3B2.1-7,
1 6
5
1
Ana M. Abrantes , Cláudio J. Maia , Maria F. Botelho 1 3 89% in HuH7 and 91% in HepG2 cell line. Regarding DNA synthesis, this value decreased
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1 Biophysics Unit, CIMAGO, Faculty of Medicine, University of Coimbra, Coimbra, 75% in Hep3B2.1-7, 66% in HepG2 and 41% in HuH7 cell line after treatment. Through
2 CICS-UBI, Health Sciences Research Centre, University of Beira Interior, Covilhã, comet assay it was observed that HepG2 tail moment (product of tail length and total DNA
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3 IBILI, Faculty of Medicine, University of Coimbra, Coimbra, Faculty of Sciences and fraction in tail) increased 64 times. The tail moment of Hep3B2.1-7 increased 3 times.
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Technology, University of Coimbra, Coimbra, Obstetrics Service, Coimbra Hospital and There is no difference between hAMPE treated and untreated HuH7 cells tail moment.
University Centre, Coimbra, Surgical Department - Surgery A, Coimbra Hospital and
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University Centre, Coimbra, Portugal Conclusions: These results show that the treatment of HCC cell lines with hAMPE
decreases the protein and DNA synthesis, as well as increases the DNA damage,
Corresponding author’s e-mail: ana_mamede@hotmail.com suggesting that hAMPE may have a promising role in the HCC therapy.

BASIC POSTER ABSTRACTS Introduction: Hepatocellular carcinoma (HCC) is a highly fatal primary liver malignancy. BASIC POSTER ABSTRACTS
HCC low survival rate depends, in part, on the resistance of this type of cancer to
conventional therapies. The potential of human amniotic membrane (hAM) in the treatment
of liver diseases has been explored in the past decade, particularly in the treatment of
hepatic cirrhosis and fibrosis. In fact, several studies indicate that in a near future hAM
may become one of the biomaterials for the treatment of liver diseases. However, until
now, there are no studies exploiting the application of hAM for the treatment of HCC.
Aims: The aim of this study is to evaluate the effect of hAM protein extracts (hAMPE)
in protein and DNA synthesis in three HCC cell lines. DNA induced-damage will be also
evaluated.
Methodology: hAM were obtained from healthy women with informed consent according
to the guidelines of Ethical Committee of Coimbra Hospital and University Centre
(Coimbra, Portugal). After the receipt, hAM were washed with phosphate buffered solution
and subjected to mechanical actions in order to extract proteins, which were quantified
using Nanodrop®. To study the effect of hAMPE in HCC, studies were performed in three
cell lines: HuH7 (mp53), HepG2 (wp53) and Hep3B2.1-7 (p53 under-expressed). Cells
were incubated with 1µg/µL of hAMPE during 72h. After this period, sulforhodamine B
and crystal violet assays were performed to assess protein and DNA synthesis. In order to
evaluate DNA damage, comet assay was carried out.