Page 106 - ebook HCC

GENEV
PROGRAMME AND ABSTRACTSAND ABSTRACTS
EASL
104 PROGRAMME GENEVA, SWITZERLANDA, SWITZERLAND EASL HCC SUMMITHCC SUMMIT 105
104
105
FEBRUAR
FEBRUARY 13 - 16, 2014Y 13 - 16, 2014
Poster Board Number B8
ASSOCIATION OF Table I: Baseline characteristics
METHYLENETETRAHYDROFOLATE REDUCTASE Characteristics Cases Control
(MTHFR) A1298C & C667T GENE POLYMORPHISM Sex (male:female) 10:1 3:1
IN HEPATOCELLULAR CARCINOMA PATIENTS Age (years) 56.55 (± 10.53) 48.1 (±10.6)
Baseline value
FROM INDIA Hb% 5.2± 21.66% 9.96 ± 2.7
ALT (IU/ml) 84.44±35.04 22.6 ± 4.9
AST (IU/ml) 107.66±35.99 69.5± 17.2
Total Billirubin 2.4 ± 0.45 1.4 ± 0.33
Dipu Bharali , Manash P. Sarma , Premashis Kar 1
1
1
1 Medicine, Maulana Azad Medical College, New Delhi, India, New Delhi, India Cigarette smoking Low dose Average
Alcohol consumption Low dose Average
Corresponding author’s e-mail: dipubhli@gmail.com Personal & Family History of None None
chronic diseases
Table II: Distribution of the genotypes and allele frequencies of MTHFR A1298C
Introduction: Hepatocellular carcinoma (HCC) is the third most frequent cause of gene polymorphism in HCC cases and controls
death in India. The most extensively studied risk factors for HCC are the association of
MTHFR1298A>C and 677C>T polymorphism. Genotype Cases (%) Control (%) P value Odd Ratio
(n=105) (n=240) (95% CI) (95% CI)
Aims: This study is designed with an aim to find out the association of A1298C & C667T MTHFR A1298C
polymorphism in HCC in comparison to liver diseases without cancer. AA (normal) 12 (11.4%) 58 (24.1%) 0.00277 0.4058 [0.2- 0.777]
BASIC POSTER ABSTRACTS Methodology: A total of 105 cases diagnosed with HCC and 240 controls enrolled from AC (risk) 9 (10.57%) 26 (8.8%) 0.269 0.772 [0.337- 1.68] BASIC POSTER ABSTRACTS

the Medicine and Gastroenterology OPD of Lok Nayak Hospital, Delhi and Madurai
Medical College, Tamil Nadu during July 2010 to September 2012. AASLD 2011 (Bruix
0.0112
1.87[1.08-3.09]
CC (risk)
80 (76.1%)
153 (63.75%)
et.al.) evidence was followed to recruit HCC cases. Total 5 ml peripheral blood samples
were collected and Genomic DNA was isolated from blood followed by amplification and
RFLP using MboII and Hinf1 enzyme for 1298AC and 677CT respectively.
Table III: Distribution of the genotypes and allele frequencies of MTHFR C677T
Results: Mean ages (±standard deviation) of HCC patients and controls were 56.55 (± gene polymorphism in HCC cases and controls
10.53) and 48.1 (±10.6) years, respectively. ALT and AST was significantly higher in HCC.
Out of 105 cases 12AA, 9AC and 80CC in comparison to 240 control (58AA, 26AC and
CC153). 1298CC are at higher risk in HCC than 1298AA. Out of 105 cases 12 CC, 58 CT Genotype Cases (%) Control (%) P value Odd Ratio
and 26TT in comparison to 240 control (17 CC, 156 CT and 63TT). 677TT genotype had (n=105) (n=240) (95% CI) (95% CI)
increased HCC risk.
MTHFR C677T
Conclusions: Current study showed MTHFR1298CC genotype (O.R =1.87; p<0.5) CC (risk) 26 (28.76%) 63 (22.25%) 0.3896 0.924[0.539- 1.562]
and 677TT genotype (OR=1.69, p<0.5) are at higher risk in HCC. A1298C and C677T CT (normal) 58 (55.23%) 156 (65.0%) 0.0445 0.665 [0.416-.064]
polymorphism may be an increased risk in HCC. The association needs further studies in
HCC patients. TT (risk) 12(11.4%) 17(7.08%) 0.0971 1.69 [0.75- 3.68]