Page 140 - ebook HCC

PROGRAMME AND ABSTRACTSAND ABSTRACTS
GENEV
EASL HCC SUMMITHCC SUMMIT
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138 PROGRAMME GENEVA, SWITZERLANDA, SWITZERLAND EASL 139
FEBRUARY 13 - 16, 2014Y 13 - 16, 2014
FEBRUAR
Poster Board Number B31
IMMUNOLEVELS OF TRANSCRIPTION FACTOR
FOXM1 AND GLYCOLYTIC ENZYME HKII
CORRELATE WITH CD90+ AND CD133+ CANCER
STEM CELLS, OXIDATIVE AND NITROSATIVE
STRESS, AND DISEASE PROGRESSION IN
HEPATOCELLULAR CARCINOMA

Lily Mei , Katherine Choi , Mamta Pant , Rohini Chennuri , Conclusions: Hepatocyte immunolevels of transcription factor FoxM1 and glycolytic
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Ana Hinojosa , Hari Sreedhar , Michael Walsh , Ming Jin , Hui Xie , enzyme HKII correlate with markers for hepatic cancer stem cells CD90 and CD133.
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Dragana Kopanja , Nissim Hay , Pradip Raychaudhuri , Grace Guzman 1 Oxidative and nitrosative stress indicators 8-OHdG and iNOS correlate with fibrosis
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1 Department of Pathology, College of Liberal Arts and Sciences, Department of and disease progression markers CK7 and CK19. CD90 correlates with increasing
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Bioengineering, Division of Epidemiology and Biostatistics, Department of Biochemistry tumor grade. These results further suggest FOXM1 and HKII play a role in promoting
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and Molecular Genetics, University of Illinois at Chicago, Chicago, United States hepatocarcinogenesis.
Corresponding author’s e-mail: lmei3@uic.edu
BASIC POSTER ABSTRACTS Introduction: Hepatocellular carcinoma (HCC) develops on a continuum of morphological BASIC POSTER ABSTRACTS
and molecular alterations in advancing chronic liver disease. FoxM1, HKII, 8-OHdG, and
iNOS have been implicated in a variety of cancers as markers for oncogenesis, increased
metabolic activity, oxidative and nitrosative stress respectively. We hypothesize that these
pro-oncogenic components act in concert to advance disease progression and influence
tumor differentiation in HCC.
Aims: To analyze immunomarkers of oncogenesis in non-dysplastic cirrhosis (NDC), liver
cell change/dysplasia in cirrhosis (LCC), HCC and normal liver controls.
Methodology: A progression liver tissue array constructed from 45 subjects with cirrhosis
and HCC, and 8 normal controls was analyzed. Standard immunohistochemistry (IHC)
was performed to determine levels of FOXM1, HKII, CD90, CD133, 8-OHdG, iNOS,
CK7 and CK19. Staining was analyzed by Aperio Image Analysis. Fisher exact test was
employed using SAS.
Results: Strong positive correlations were found between various IHC stains and disease
progression (Table 1). Tumor grade also correlated with CD90 hepatocyte cytoplasmic
staining (CD90HS).