Page 166 - AAOMP Onsite Booklet

2018 Joint IAOP - AAOMP Meeting

#138 AN IMMUNOHISTOCHEMICAL ANALYSIS OF LYMPHOCYTIC
INFILTRATION IN FOLLICULAR ADENOMA AND PAPILLARY

CARCINOMA THYROID; RELATION TO PROGNOSTIC VARIABLES

Monday, 25th June - 00:00 - Poster Session Available from 25th (16:30- 18:30) -26th (18:30-20:30) June 2018 -
Bayshore Ballroom D-F - Oral to Poster - Abstract ID: 187

Dr. Varda Jalil (superior university), Dr. rabia safdar (Uni), Prof. Abdul Hanan Nagi (retired (ex prof of university of health sciences,
Lahore))

OBJECTIVE
To determine the density of CD 8+ve Cytotoxic T Lymphocytes (CTLs) & CD 56+ve Natural Killer (NK) cells in
biopsies of patients with follicular adenoma and papillary thyroid carcinoma (PTC)

FINDINGS
50 diagnosed paraffin embedded tissue blocks of follicular adenoma and papillary carcinoma thyroid were recruited
according to the exclusion and inclusion criteria. Density of CD 8 +ve CTLs & CD 56 +ve NK cells was determined
immunohistochemically and related with other histological parameters. The density of CTLs & NK cells was scored
from 1–5 by French et al 2010. According to it, Score 1 is Single lymphocytes in the peritumoral region, Score 2
indicates Lymphocyte aggregates in the peritumoral region, Score 3 shows Intratumoral single lymphocytes with or
without lymphocytes in the peritumoral region, Score 4 shows Fewer than 10 intratumoral aggregates while Score 5
indicates 10 or more intratumoral aggregates with or without peritumoral lymphocytes. Score 5 indicates extensive
infiltration. Increased frequency of score 3 (56%) and score 5 (40%) were observed in cases of follicular adenoma
and PTC respectively (p=0.01). Whereas when the density of CD 56 +ve NK cells was related to the total number of
follicular adenoma and PTC, increased frequency of score 3 (96%) was observed in cases of follicular adenoma as
compared to PTC (52%) (p=0.000)
CONCLUSION
The density of peritumoral & intratumoral lymphocytic infiltrate varies with transition from benign to malignant
tumour. The prevalence of both CD8+ve T cells and CD+ve 56 NK cells are important in both follicular adenoma and
papillary carcinoma of thyroid. Tumour infiltration by CD 8+ve cytotoxic T cells increases while CD 56+ve NK cells
decreases asit progresses from benign to malignant neoplasm. It might be an independent prognostic factor if
studied on a comparatively large sample size with follow up of the patients.

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