Page 86 - JWP 120122

86 Mohammad Naufal Mohammad Shah, Khairul Bariah Mohd Johan, Azuan Roslan, Hasrul Zaman
Hassan Basri, Elizabeth Pesiu, Muhammad Aidil Zahidin, Mohd Tajuddin Abdullah & Mohamed Nor
78 Mohammad Naufal Mohammad Shah, Khairul Bariah Mohd Johan, Azuan Roslan, Hasrul
Zalipah
Zaman Hassan Basri, Elizabeth Pesiu, Muhammad Aidil Zahidin, Mohd Tajuddin Abdullah &
Mohamed Nor Zalipah

Location of
Figure 1. Location of the three sampling sites in the state of Terengganu,
located on the east-coast of Peninsular Malaysia (inset map). The study sites are:
A) Gunung Tebu Forest Reserve, B) Sungai Buweh, Kenyir, C) Saok Waterfall,
Kenyir.

In the laboratory, centrifuge tubes containing 1 ml ethanol preserving the pollen
swabs were manually shaken prior to micropipette extraction. 1 µl of ethanol was
then pipetted and put on a glass slide for observation under a light microscope
(Leica DME) at 40x magnifications. A Dino-eye microscope eyepiece camera
(AM423X, Taiwan) was used to aid pollen identification with a maximum
magnification of 2800x. Identification of the pollen grains was conducted by
referring to Mohamed (2014). For each sample, the same steps were repeated 10
times, thus pollen identification was conducted from 10 µl of ethanol for
maximum pollen detection. For the seed identification, the dried faecal samples
were immersed in distilled water for approximately 24 hours to separate the seeds
from the faecal material. The mixture was then filtered using filter paper for
observation under a dissecting microscope with x1 to x4 magnification. We
compared the list of plant species previously reported to be pollinated and the
seeds dispersed by the pteropodid bats in Peninsular Malaysia by Hodgkison