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Diagnostic Laparoscopy  107

                                  in fact not become evident for several hours, as volume expansion with
                                  fluid shifted from the extravascular space takes approximately 4 h, and
                                  splenic  recruitment  of  red  blood  cells  offsets  the  fall  in  packed  cell
                                  volume  even  longer  (Villiers,  2005).  When  the  blood  loss  is  less  than
                                  20%, crystalloids will suffice, whereas if the blood loss is between 20
                                  and 30% colloids are necessary. Blood transfusion should be considered
                                  if the blood loss approaches 30%, as physiological mechanisms are no
                                  longer able to compensate.
                                    Carbon dioxide embolism presents with a severe and sudden drop in
                                  blood pressure, development of a heart murmur, cardiac dysrhythmias
                                  and cyanosis. A rapid and transient rise in end-tidal carbon dioxide as
                                  the gas embolises is followed by a steep decline in this parameter, due to
                                  reduction of blood delivery to the pulmonary circulation (Staffieri et al.,
                                  2007). Capnography, together with ECG and blood-pressure monitor-
                                  ing, will assist in rapidly detecting carbon dioxide embolism.
                                    An accidental perforation of the diaphragm, or a pre-existent congeni-
                                  tal or acquired defect, may cause pneumothorax, which manifests itself
                                  with a change in ventilator pattern, accompanied by cyanosis, drop in
                                  PaO2 and caudal bulging of the diaphragm. Due to the positive perito-
                                  neal pressure, a tension pneumothorax will develop, which may be fatal
                                  if not rapidly recognised and treated. Increase in the minute ventilation,
                                  evacuation of the gas from the thorax with a thoracostomy tube, and
                                  abdominal desufflation are able to reverse the physiological effects while
                                  the laceration in the diaphragm is repaired. Conversion to open surgery
                                  is often necessary.
                                    Postoperative care of animals undergoing laparoscopy includes multi-
                                  modal  analgesia,  with  parenterally  administered  opioids,  and  non-
                                  steroidal  anti-inflammatory  agents  whenever  possible.  Although  the
                                  discomfort after laparoscopy is mostly due to formation of carbonic acid
                                  on serosal surfaces, causing peritoneal inflammation and phrenic nerve
                                  irritation  rather  than  incision-related  pain  (Magne  and  Tams,  1999),
                                  injection of local anaesthetic agents at the port sites at the beginning or
                                  at the end of the procedure is considered beneficial. Long-acting local
                                  anaesthetic agents such as bupivacaine or ropivacaine are always used;
                                  as their onset of action is delayed, a cocktail with lidocaine is preferred
                                  when the block is carried out at the end.
                                    In preparation for laparoscopy the bladder should be emptied to mini-
                                  mise the risk of accidental puncture with the Veress needle or a trocar.
                                  The stomach and occasionally colon may need to be evacuated if they
                                  are distended. Distended viscera will also decrease visualisation of the
                                  surrounding areas.
                                    The animal should always be clipped liberally, from 5 cm cranial to
                                  the xiphoid process to the pubis. Laterally, the patient is clipped as for
                                  a traditional open coeliotomy, and the whole clipped area is prepared
                                  aseptically.  This  will  enable  conversion  to  an  open  procedure  should
                                  the necessity arise. This may happen because of technical difficulties or
                                  complications, or in case a surgically correctable lesion is encountered.
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