Page 122 - Clinical Manual of Small Animal Endosurgery
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110   Clinical Manual of Small Animal Endosurgery

             Surgical technique

                              The first step in laparoscopy is induction of pneumoperitoneum. This
                              can be achieved with the use of either a Veress needle (closed technique)
                              or  a  Hasson  trocar  (open  or  paediatric  technique).  Palpation  of  the
                              abdominal cavity is recommended before needle insertion, in order to
                              identify underlying organs – most commonly the spleen – which must be
                              avoided. The Veress needle is usually inserted at the same site as the first
                              telescope portal; this is often located caudolateral to the umbilicus, where
                              the abdominal wall is consistently thin (Kolata and Freeman, 1999a). A
                              1 mm skin incision is performed at the selected site, and the abdominal
                              wall is lifted and tented by grasping it with forceps. The Veress needle
                              is then inserted directed caudally at an angle with the skin, thus avoiding
                              the spleen and the falciform ligament. Other measures directed at mini-
                              mising risk of iatrogenic organ damage are placing the animal with the
                              head slightly down (Trendelenburg position), and resting the heel of the
                              non-dominant hand on the abdominal wall, to control the needle entry.
                                The Veress needle is grasped by the hub during abdominal wall inser-
                              tion, thus allowing retraction of the inner blunt stylet. Once the abdomi-
                              nal cavity is entered, and no more resistance is encountered, the outer
                              trocar retracts, and the spring-loaded blunt stylet protrudes, minimising
                              the risk of trauma to the abdominal organs. After insertion, the needle
                              is  gently  swept  in  a  circular  pattern  against  the  abdominal  wall,  thus
                              freeing it from any adhesions or omentum.
                                To verify appropriate intra-abdominal placement, the ‘hanging drop
                              test’ is performed. This entails attaching a syringe partially filled with
                              saline to the hub of the Veress needle. After having applied gentle suction
                              to confirm the absence of blood or fluids, a drop of saline is placed into
                              the hub of the needle, and the abdominal wall is tented. If the needle is
                              properly placed in the peritoneal cavity the negative pressure present in
                              the  abdominal  cavity  aspirates  the  drop  into  the  needle  hub.  Correct
                              placement is extremely important, as placement into an organ, vessel or
                              mass causes haemorrhage, which interferes with visualisation, and can
                              also result in a fatal embolism.
                                The needle can also be incorrectly placed into the subcutaneous tissue,
                              or  into  the  omentum  or  falciform  ligament.  Subcutaneous  placement
                              results  in  subcutaneous  emphysema,  which  will  greatly  increase  the
                              difficulty  of  the  procedure.  Subcutaneous  emphysema  will  resolve  in
                              approximately  48 h.  Similarly,  insufflating  carbon  dioxide  below  the
                              omentum  or  within  the  falciform  ligament  causes  expansion  of  these
                              structures, and consequent obscuration of the field of view.
                                Once  correct  placement  of  the  needle  is  confirmed,  the  Luer-lock
                              attachment  at  the  hub  of  the  needle  is  connected  to  the  insufflation
                              tubing,  and  pneumoperitoneum  is  established  (Fig.  4.4).  The  pressure
                              within the abdominal cavity should be initially low (2–3 mmHg), and
                              carbon dioxide should be delivered at a rate of at least 1 L/min. Greater
                              intra-abdominal pressure, or a flow rate close to zero, are suggestive of
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