Page 28 - The Veterinary Laboratory and Field Manual 3rd Edition

List of figures, plates and tables xxvii

Plate 18 Real-time PCR amplification curves and Plate 35 Section of brain under ultraviolet light.
the melting peaks. Fluorescent sections stand out.
Plate 19 LAMP isothermal amplification products Plate 36 There are often a lot of deaths at
can be visualized using naked eye due to the lambing time but the presence of the occasional
accumulation of PCR by product magnesium deformed lamb may not necessarily indicate that
pyrophospahte (cloudy) or colour change using there is an infectious disease present. Toxins can
SYBR green. also cause deformities.
Plate 20 A representative of sequencing output. Plate 37 Skin rash seen in a pig with
Plate 21 Phylogenetic Tree of a total of over 180 Erysipelothrix insidiosa sp.
partial σC gene sequences of avian reovirus. Plate 38 Histology section H&E 20× of a normal
Plate 22a Blood smear from a cow which later chicken (Gallus gallus) lung.
died following fever, haematuria and weight Plate 39 Histology section H&E 20× of a bird
loss over a period of several days. Giemsa 20x intestine (chicken, Gallus gallus) illustrating
magnification. haemorrhage secondary to a bacterial infection.
Plate 22b Poor quality bovine blood smear Plate 40 Histology section of a bird liver
stained with Giemsa 100× oil immersion. 40× stained with Perl’s Prussian Blue iron
Plate 23 Equine blood smear viewed under stain to illustrate the presence of iron stored
oil immersion (Diff Quick 1000×) illustrating as hemosiderin in hepatocytes and Kupffer
numerous polymorph neutrophils (N), an (macrophage) cells.
eosinophil (E) and a basophil (B). Diff Quick™ Plate 41 Illustration of gross necropsy on a
stain. RBC are also present. freshly dead aviary bird (parakeet) illustrating
Plate 24 Equine blood smear viewed under oil an enlarged and discoloured liver with several
immersion 1000× illustrating granulocytes (E an N) abscesses.
and agranulocytes (M and L). Plate 42 The use of immune-histochemistry
Plate 25 Canine band neutrophil (immature to identify lesions caused by Yersinia
PMN). pseudotuberculosis (serotype 3) in the liver of the
Plate 26 Feline eosinophil. case illustrated in Plate 41.
Plate 27 Feline basophil. Plate 43 Histology section of a wild bird liver
Plate 28 Feline blood smear showing rouleaux (New Zealand kokako [Callaeas cinereal]) 10×
formation. stained with Perl’s Prussian Blue iron stain to
Plate 29 Immune response generated following illustrate the presence of excess stored iron.
exposure to a pathogen can be innate (non-specific) Plate 44 (A) A hunted muskox cow with a
response and adaptive (specific) response. sampling kit that will be used by the hunter
Plate 30 Serum antibody concentrations following to collect a set of biological samples when
primary and secondary infections. butchering the carcass. (B) The core set of
Plate 31 Photograph of a plate used to perform samples collected using the sampling kit:
the haemagglutination inhibition test. blood-saturated filter-paper strips, faeces, left
Plate 32 Diagrammatic representation of the metatarsus (or left hind leg), and a piece of skin
haemagglutination inhibition test. with hair from the rump.
Plate 33 Glass slides with organophosphate Plate 45 Bovine cerebral cortex.
positive and negative serum samples are shown Plate 46 Abdomen, cat. Acute inflammation.
in the insert. Plate 47 Thorax, bovine with chronic suppurative
Plate 34 A formalin fixed bovine brain with bronchopneumonia.
polioencephalomalacia in the grey matter. Plate 48 Liver, sheep. Necrosis.

Vet Lab.indb 27 26/03/2019 10:25