Page 24 - The Veterinary Laboratory and Field Manual 3rd Edition

List of figures, plates and tables xxiii

Figure 5.5 (a) Microhaematocrit reader. Figure 6.6 Diagrammatic representation of
(b) Microhaematocrit centrifuge. 293 what can be seen in the slide agglutination
Figure 5.6 Blood smear from a cow test. 311
which later died following fever, haematuria Figure 6.7(a) Tube agglutination test with
and weight loss over a period of several doubling dilutions beginning at 1 in 5. 312
days. 295 Figure 6.7(b) The quantitative precipitin test. 313
Figure 5.7 Poor quality bovine blood smear Figure 6.8(a) Typical layout used for an
stained with Giemsa 100× oil immersion. 295 ‘Ochtelony’ diffusion test. 315
Figure 5.8 Equine blood smear viewed under Figure 6.8(b) Agar gel immunodiffusion test. 315
oil immersion (Diff Quick 1000×) illustrating Figure 6.8(c) Agar gel immunodiffusion is
numerous polymorph neutrophils (N), an a qualitative test is used for the detection of
eosinophil (E) and a basophil (B). 296 antibodies against influenza A virus routinely. 316
Figure 5.9 Equine blood smear viewed Figure 6.9 (a) Photograph of a plate used to
under oil immersion 1000× illustrating perform the HI test (note that this is not the
granulocytes (E and N) and agranulocytes same test plate as that shown in Figure 6.9b). 317
(M and L). (E) Eosinophil, (N) polymorph Figure 6.10 Principle of the CFT. 319
neutrophil. 296 Figure 6.11 Fluorescent antibody technique/
Figure 5.10 (a) Canine band neutrophil immunofluorescence. 320
(immature PMN). (b) Feline eosinophil. Figure 6.12 (a) Immunocytochemical
Note reddish granules in the cytoplasm and staining of a section of liver (20×) from a bird
segmented nucleus. (c) Feline basophil. that died following infection with Yersinia
(d) Feline blood smear showing rouleaux pseudotuberculosis. (b) Immunocytochemical
formation. 297 staining of a section of intestine from a dog
Figure 6.1 (a) Immune response generated which died following infection with parvo viral
following exposure to a pathogen can be infection. 321
innate (non-specific) response and adaptive Figure 6.13 (a) ELISA technique for
(specific) response. (b) Innate immune response measuring the amount of antigen in a test
leads to antigen presentation and subsequent sample (indirect method).
development of adaptive response. 301 (b) ELISA technique for measuring the amount
Figure 6.2(a) Diagrammatic representation of of antibody in a test serum (indirect method). 323
some aspects of phagocytosis, which is part Figure 7.1 Chemstrip® uG/K are test
of the innate immune response. 302 strips used in human medicine for the semi-
Figure 6.2(b) Essential features of the quantitative determination of glucose in urine
immune response. 303 and for the detection of ketone bodies
Figure 6.3 A simple chart outlining the cells (for example, acetone) in urine. 331
involved in the specific immune response Figure 7.2 The principle of wavelength. 332
which consists of (1) humoral response and Figure 7.3 The electromagnetic spectrum. 333
(2) cell-mediated response. 304 Figure 7.4 The visible part of the
Figure 6.4(a) The structures of classes of electromagnetic spectrum. 333
antibodies. 306 Figure 7.5 Light reflected, absorbed and
Figure 6.4(b) Antigen–antibody binding – transmitted when it falls on a coloured
precipitation. 307 solution. 334
Figure 6.5 Serum antibody concentrations Figure 7.6 Calibration graph showing the
following primary and secondary infections. 308 potential linear or exponential relationship

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