Page 20 - The Veterinary Laboratory and Field Manual 3rd Edition

List of figures, plates and tables xix

Figure 2.36 Analytical balance. 71 Figure 2.65 Pipette volumetric. 96
Figure 2.37 Water bath. 73 Figure 2.66 Pipette graduated. 97
Figure 2.38 Deionizer. 75 Figure 2.67 Pipette volumetric close up. 97
Figure 2.39 Manesty water distillation Figure 2.68 Pipette filler. 98
apparatus. 75 Figure 2.69 Pipette filler electric. 98
Figure 2.40 Air-displacement pipette. 76 Figure 2.70 Ultrasonic bath. 103
Figure 2.41 Positive displacement pipette. 76 Figure 3.1 Various plastic bottles and bags
Figure 2.42 Micropipette. 77 may be suitable for collecting faecal samples. 115
Figure 2.43 Dispenser. 79 Figure 3.2 The use of a plastic bag to protect
Figure 2.44 Semi-automatic photometer, hands from direct contact with faecal material
Rayto RT-9200 (wavelengths in the visible if waterproof gloves are not available. 116
spectrum between 330–800 nm, five standard Figure 3.3 A temperature controlled
filters, three optional filters, flow cell. 79 centrifuge with an Eppendorf rotor. 117
Figure 2.45 Diaspect Tm handheld Figure 3.4 Larval identification is difficult but
haemoglobinometer. 80 some common species may be recognized by
Figure 2.46 Parts of the microscope, the characteristic length and shape of the tail
Olympus CX43. 81 as well as the number of cells in the intestinal
Figure 2.47 Parts of the microscope, Leica tract. 118
DM4. 82 Figure 3.5 Filling the chambers on a
Figure 2.48 The microscopic field is divided McMaster slide (A) for a worm egg count
according to the plate of a clock. 85 (see text). A fine tipped pipette (B) is used
Figure 2.49 Systematic examination of the to fill the counting chamber(s) of the slide
microscopic field. 86 (C) with faecal suspension. 120
Figure 2.50 Working principle of an oil Figure 3.6 Flotation: (A) weighing 4 g of
immersion objective. 87 faecal material; (B) dilution of faecal material
Figure 2.51 ELISA reader using filters. 88 in water and filtering through a gauze pad into
Figure 2.52 ELISA plate washing station. 88 centrifuge tube; (C) filling of centrifuge tubes to
Figure 2.53 ELISA reader with a equal level; (D) diluting faecal pellet in Sheath
monochromator (filters no longer needed). 88 solution after centrifugation; (E) filling tubes
Figure 2.54 RT PCR cycler, safety cabinet and enough to form meniscus, cover tubes with
no-break-system. 89 cover slips; (F) remove cover slip from tube
Figure 2.55 Sysmex pocH-100iV Diff and transfer to labelled microscope slide. 121
haematology analyser. 89 Figure 3.7 The Baermann equipment (A) used
Figure 2.56 Sysmex pocH-100iV Diff screen. 90 for extracting lungworm and an illustration of a
Figure 2.57 Standard glassware store lungworm larva (B). A sieve (250 µm) is placed
containing flasks and glass bottles. 91 in the wide neck of a glass funnel held in a
Figure 2.58 Measuring volumes, reading the retort stand. 122
meniscus. 93 Figure 3.8 The general topography of the
Figure 2.59 Glass reagent bottles. 94 bovine abdomen to show the location of the
Figure 2.60 Beakers. 94 gut in situ. 125
Figure 2.61 Erlenmeyer flask. 95 Figure 3.9 Simple plan of the ruminant
Figure 2.62 Measuring flask. 95 intestinal tract. 125
Figure 2.63 Measuring cylinder. 95 Figure 3.10 Detailed anatomical outline of
Figure 2.64 Pipette volumetric bunch. 96 the intestines of a cow. 125

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