Page 22 - The Veterinary Laboratory and Field Manual 3rd Edition

List of figures, plates and tables xxi

Figure 3.46 Dorsal view of two species of laboratory at a district veterinary diagnostic
adult louse (insects – three pairs of legs). 192 facility. 217
Figure 3.47 Lateral view of a flea. (C) Figure 4.11a and b Primary biochemical
(Ctenocephalides sp.) cat and dog fleas. 193 tests for (a) Gram +ve and (b) Gram –ve
Figure 3.48a Typical louse life cycle in which bacteria. 221
there is no metamorphosis. 195 Figure 4.11c Simple biochemical screening
Figure 3.48b Typical fly life cycle test (TSI slopes) can be used to determine
characterized by metamorphosis. 195 the species of selected bacteria during survey
Figure 3.49a The life cycle of the equine work but in most cases a series of 20–30 tests
stomach bot (Gasterophilus intestinalis). 196 will be required. 222
Figure 3.49b Horse stomach opened out to Figure 4.11d API strip card used to illustrate
illustrate the appearance of the larvae of the the typical biochemical reactions of ‘type’
horse bot fly (Gasterophilus sp.). 196 cultures representative of bacterial species
Figure 4.1 The relative size of a red cell, obtained from the American Type Culture
a streptococcal bacterium, a chlamydial agent Collection. 224
and an adenovirus. 199 Figure 4.12 The dilution technique can be
Figure 4.2 The principal structures of a used to count bacteria (see text). 225
bacterial cell. 200 Figure 4.13a Factors affecting the choice of
Figure 4.3 Scanning electron micrograph an antibacterial drug. 226
(SEM) of a gram-negative bacterium Figure 4.13b Antibiotic sensitivity testing. 230
(Yersinia sp.). 201 Figure 4.13c and d Antibiotic sensitivity
Figure 4.4 Microscopic appearance of testing plates. 230
rod shaped bacteria as seen using an oil Figure 4.14a The morphology of yeasts and
immersion lens (1000×). 202 fungi; this figure demonstrates some of the
Figure 4.5 Agar plates showing culture morphological features of yeasts and the
media and growth characteristics of colonies species of fungi (Penicillium spp. and
of different microorganisms. 209 Aspergillus sp.). 235
Figure 4.6 (a) Anaerobic jar with sealed lid Figure 4.14b Diagrammatic representation
(A) and a GasPak (B). (b) This is an example of of some fungal species. 236
a chamber used for growth of anaerobic Figure 4.15 Steps in viral disease diagnosis
bacteria. 210 starting from the evaluation of clinical signs
Figure 4.7 Classical microbiology requires a leading to laboratory confirmation. 241
wide range of reagents and relies on the Figure 4.16 Modern laboratory assays
technical expertise and experience of the are available targeting various structural
laboratory technician for the successful components of the enveloped and naked viral
culture and identification of disease causing particles. 241
agents. 211 Figure 4.17 Identification and isolation of
Figure 4.8 Initial ‘streaking’ of a culture plate viruses from clinical samples. 242
and preparation of a subculture (or ‘purity’ Figure 4.18a Canine airway epithelium
plate). 215 with intracytoplasmic inclusions of canine
Figure 4.9 Classification chart according to distemper virus (the arrow indicates
staining reaction and cellular morphology of eosinophilic intracytoplasmic inclusion bodies). 244
common bacteria of veterinary importance. 216 Figure 4.18b Trachea of a chicken infected
Figure 4.10 Sink in a small microbiology with infectious laryngotracheitis virus with

Vet Lab.indb 21 26/03/2019 10:24