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250                Natural Antioxidants: Applications in Foods of Animal Origin
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                              Colorimetry Frozen Restructured Beef Steaks
                                      95% Cl  for the Mean
                    26
                    22  I
                    24
               ro
               'iii
              0     20
              *  ro
               c    18
               (;)   16
               E
              ·g    14
              0                     I     I     I     I  I
              u     12         I
                    10                                            I    I






              Minolta CR300 colorimeter C light source 8mm aperature

            FIGURE 6.35  Colorimetry data (a*) complement GCMS and sensory data. Raw meat color
            of frozen restructured steaks in non-barrier packaging decreases over time. The data suggest
            when raw meat color is a critical consumer attribute, barrier packaging may be required.



            6.3  MEASURING THE CONSEQUENCES OF LIPID OXIDATION

            6.3.1  WATER HOLDING CAPACITY (WHC)

            Consequences of lipid oxidation is not limited to flavor, aroma, and color,
            WHC is also adversely affected (Figure 6.36). As WHC decreases during
            frozen storage, meat products will lose moisture during cooking resulting in
            dry, “tough,” and chewy steaks.
               Studies  (Lund  et  al.,  2007)  conducted  by  meat  scientists have  deter-
            mined that myofibrillar proteins are susceptible to chemical modification
            due to oxidation. It is myofibrillar protein oxidation that has been a factor
            in observed loss of WHC in raw and cooked value-added meats. Our study
            was designed to determine the effect of natural antioxidants on meat protein
            oxidation in raw frozen restructured beef steaks during 90 days of frozen
            (–10 °F, –23 °C) storage. After 30, 60, and 90 days of frozen storage the
            restructured steaks were thawed at 38 °F (6 °C) overnight and evaluated for
            raw meat color (oxidation of sarcoplasmic protein) and WHC (a measure of
            myofibrillar protein function). The trend in the data shows WHC decreases
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