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204    Tasks for the Veterinary Assistant


          the seal on the needle, set it down or tuck it between
          your ring finger and little finger with the top up so the
          syringe doesn’t fall out. Use your index fingers and
          thumbs to break the seal on the needle by holding the
          long part of the needle between the fingers of one hand
          and twisting the short top with the others. Be careful not
          to touch the hub with your fingers or hand. Pick up the
          syringe case and turn it so as it drops you can catch the
          plunger end with your fingers. Be careful not to touch
          the tip of the syringe with your bare hand or fingers.
          Hold the needle between your index finger and thumb
          and carefully slide the tip of the syringe into the needle
          hub. Leave the cap on the needle at this point.


          Solution Bottle Preparation                        FIGURE 11.30  Inserting a needle into a bottle.

          If using a multiple sample bottle, a bottle that will be
          used on multiple patients, it is good practice to swab
          the rubber stopper top of the solution vial or bottle
          with an alcohol‐soaked cotton ball. The only exception
          would be if it is a single‐dose vial that has come right
          out of the storage container or if the solution in the
          bottle is a modified or live vaccine. It should say on the
          bottle or box.
            Remove the needle cap and draw an amount of air
          into the syringe equal to the amount of solution to be
          withdrawn from the vial. With the bevel of the needle
          pointing up, you should be able to see the lumen. Pick
          up the bottle with your non‐dominant hand and hold it
          with the stopper pointing down (Figure 11.30). Insert
          the needle through the rubber stopper with a slight
          backward pressure on the beveled side of the needle to
          prevent coring of the rubber stopper. Always try to center
          the needle in the top of the stopper. Continue to hold   FIGURE 11.31  Ridding the syringe of air bubbles.
          the  bottle  upside  down  so  the  solution  falls  into  the   immediately on the patient. Patient name, drug, and
          center. Depress the plunger to inject the air into the vial.   route should be listed on the tape and put in a spot that
          This prevents pressure from building up in the bottle   doesn’t cover the graduations. If you must, tear the tape
          which in time will make the solution shoot out of the vial   in half lengthwise to give you a thinner piece. A second
          through the holes in the rubber stopper. To withdraw   technique is to mark the barrel opposite of the gradua-
          the solution, pull down on the plunger drawing the fluid   tions with a permanent marker.
          into the syringe. Draw a milliliter or more solution than
          you need in order to help eliminate air bubbles. Hold
          the inverted bottle between your index finger and  Preparing Vaccines
          thumb, let the syringe rest against the ball of your palm
          (Figure  11.31). Then eliminate large air bubbles by   Some vaccines come as a powder and need to be recon-
          tapping or flicking the syringe with your other fingers to   stituted with a diluent which can be sterile water or a
          make them rise toward the tip of the syringe. Slowly   liquid vaccine that is formulated to be mixed with the
          depress the plunger to inject the air bubbles and excess   powder (Figure 11.32). The most common of these are
          solution back into the bottle until you reach the proper   the feline and canine distemper vaccines. They are pack-
          dosage of medication as prescribed by the veterinarian.   aged in trays with one powder for every one diluent.
          Withdraw the needle from the vial and recap the needle   Always read the packaging to make sure you are getting
          by sliding the needle into the cap lying on the table.   the appropriate vaccine for each species.
          Never pick the cap up to recap the needle, this is to avoid   Assemble a needle and syringe, gather one of each
          being inadvertently poked by the needle. Label the   vial. Insert the needle into the diluent bottle just inside
          syringe with a piece of tape if it’s not going to be used   the stopper in order to aspirate all the fluid, there should
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