Page 197 - The Manga Guide to Biochemistry
P. 197

Is this right?

Reaction rate
   (pmol/hour)

                                                    substrate              You got it. Nice
                                           concentration (µM)                curve, Kumi!*

* It's rare to get a smooth curve like this from real-world measurements.
   This is just the ideal shape.

                                        Although we'll use this data
                                       to figure out Vmax and Km for

                                                   DNA polymerase...

                we can't actually determine                                WHAT?!
                    those from this curve!

                    To get the values we need, we
                  have to create a graph called a
                Lineweaver-Burk reciprocal plot.

                   Line...                     Those are just
                weaver...                    the names of the

                  what?                       guys who came
                                                    up with it.
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