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Enzymes in Tropical Soils   15


                           those that involve in the cycles of N, P, and S, which are recently most extensively
                           investigated,  i.e.  urease,  phosphatases,  and  arylsulfatase.  The  presence  of
                           phosphatases  and  arylsulphatases  are  expected  because  these  soil  enzymes
                           accelerate the decomposition of organic P and organic S, respectively, which are
                           not available for plant root absorption, to release inorganic P and S such as H 2 PO 4   -
                                         2-
                                 2-
                           or HPO 4  and SO 4 , that are available for plant root absorption (Tabatabai, 1982;
                           Rojo et al., 1990).  Unlike phosphatases and arylsulfatases, the presence of urease
                           in soils is not expected.  Urease may waste the use of Urea-N because its presence
                                                                              +
                           increases the hydrolysis of Urea to produce ammonium (NH 4 ).  Ammonium may
                                                        -
                           encounter nitrification to form NO 3  in an oxydative condition or denitrification to
                           form N 2  in a reductive condition.  Nitrate-N molecules are mobile in soils or may
                           volatilize to the atmosphere.  Therefore, the presence of urease is usually inhibited
                           by Urease Inhibitors (Broadbent et al., 1985; Hendrickson and O’Connor, 1987; Cai
                           et al., 1989; Zhao and Zhou, 1991; Hendrickson and Doughlass, 1993; Sanz-Cobena
                           et al., 2008).
                                The  presence  of  enzymes  like  phosphatases  and  arylsulphatase  may  then
                           increase  the  availabilities  of  some  nutrient  elements  from  non-mineral  sources.
                           The  accumulated  organic  matters  in  the  environment,  which  contain  some
                           important nutrient elements, may be used as nutrient element sources.  This may,
                           in  turn,  lower  the  need  of  nutrients  that  are  usually  supplied  by  commercial
                           fertilizers.  The optimation of the environmental properties may be regulated to
                           manage  the  production  of  soil  enzymes  so  that  parts  of  the  nutrient  elements
                           needed by plants can be supplied from organic sources.


                           2.1  Definition and Properties of Enzymes

                                Enzymes  are  catalysts.  A  catalyst  accelerates  the  rate  of  a  reaction  to  a
                           magnitude  of  several  orders.  Enzymes  are  biocatalysts  that  are  able  to  enhance
                           biochemical reactions.  Soil enzymes are the biocatalysts working on biochemical
                           reactions in the soil system.  For example, phosphatase is a biocatalyst working on
                           the  biochemical  reaction  that  decomposes  soil  organic  P  to  produce
                           orthophosphates.  Without the presence of phosphatase, the decomposition of soil
                           organic P occurs relatively slowly.  This may result in a slow supply of phosphorus
                           available for plant root absorption and plants are more dependent on the supplies
                           of P from other sources.
                                Tabatabai (1982) stated that the most remarkable properties of enzymes are
                           their: (a) catalytic efficiency and (b) specificity.  An enzyme may efficiently enhance


                                                                          Abdul Kadir Salam - 2014
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