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Enzymes in Tropical Soils   21



                           where x is the concentration of the converted substrate.



                           2.5  First Order Enzyme Reaction

                                Unlike  that  at  high  concentration  of  substrate,  the  rate  of  an  enzymatic
                           reaction  at  any  time  at  lower  concentration  of  substrate  is  dependent  on  the
                           concentration of substrate following the first order enzyme reaction.  The rate of a
                           reaction increases with the increase in substrate concentration as shown in Fig. 2.3.
                           The rate of the biochemical reaction at low substrate concentration is controlled by
                           Eq. 2.7 as follows (Tabatabai, 1982; Sparks, 1989).


                                       (     )   .......   Eq. 2.7


                           where a is the initial concentration of substrate, x is the concentration of converted
                           substrate, t is the unit of time (in minutes), and k is the rate constant.  The value of
                           a is higher than or equals to x.  The concentration of a decreases while that of x
                           increases with time.  The value of x increases with time approaching the value of a,
                           at which the reaction will cease.
                                By integrating Eq 2.7, Eq 2.8 and Eq. 2.9 are obtained (Tabatabai, 1982).


                                             .......   Eq. 2.8


                           or


                                                 .......   Eq. 2.9


                           These equations clearly show that the rate of an enzymatic reaction is dependent
                           on the concentration of substrate and that of the converted substrate as well as
                           time.   The rate constant increases  with time approaching V max  in relation to the
                           decrease in the substrate left in the system.
                                Rearranging Eq. 2.9 results in Eq 2.10 as follows (Sparks, 1989).


                                       (     )                   .......   Eq. 2.10


                                                                          Abdul Kadir Salam - 2014
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