Page 62 - ASME_NEMB_2016_Program

Technical Program TRACK 5

TUESDAY, FEBRUARY, 23 cation, PolyDOTS were created using the donor-acceptor conjugated poly-
mer, polycyclopentadithiophene benzoselenadiazole (PCPDTBSe), which
has tunable optical properties correlating to the molecular weight of the
5-2 polymer. Various molecular weight fractions were combined at specific ratios
NANOMATERIAL PROPERTIES AND SENSING APPLICATIONS to develop a hybrid nanoparticle capable of both fluorescence imaging and
heat generation upon exposure to light. PolyDOTS have an approximate
hydrodynamic diameter of 90nm and stable fluorescence following multiple
Harris 11:30am - 1:10pm heating/ cooling cycles, as well as minimal changes in the optical properties
after a 30day exposure to ambient light. Upon exposure to 800nm light,
PolyDOTS generated a 42 degrees Celsius temperature increase above
11:30am Polydispersity Of Gold Nanoparticles: A New Metric For ambient temperature. PolyDOTS were evaluated as effective photother-
Quantitative Photothermal Conversion In Biomedical Applications mal agents against breast epithelial cells. Minimal cytotoxic response was
observed in the absence of infrared light. In the presence of 800nm light,
Technical Presentation. NEMB2016-6109 PolyDOTS incubated with MDA MB 231 breast cancer and MCF10A non-tu-
morigenic cells generate significant heating, resulting in a 90% decrease in
Zhenpeng Qin, University of Texas at Dallas, Richardson, TX, Unit- cell viability at concentrations of 50ug/ml and higher. Fluorescence micros-
copy revealed that PolyDOTS localized throughout the cell but were exclud-
ed States, Yiru Wang, University of Minnesota, Minneapolis, MN, ed from the nucleus, and no photobleaching occurred during prolonged
United States, Jaona RANDRIANALISOA, GRESPI University of imaging. These results demonstrate that PolyDOTS represent a new class
Reims Champagne-Ardenne, Reims,France, Vahid Raeesi, Warren of theranostic nanoparticles built from the platform of conjugated polymers
Chan, University of Toronto, Toronto, ON, Canada, Wojciech Lip- by selectively combining fluorescent and heat generating polymers into one
inski, The Australian National University, Canberra,Australia, John stable nanoparticle that shows no fluorescence quenching and excellent
Bischof, Univ Of Minnesota, Minneapolis, MN, United States photothermal capacity.

Gold nanoparticles (GNPs, including sphere, rod, and shell etc.) are increas- 12:10pm Dual-functional moiré metasurfaces for nanospectros-
ingly used for biomedical applications due to unique optical properties, copy and phototherapy
established synthesis methods, and biological compatibility. Despite recent
developments in using GNP plasmonic heating for thermal therapy, imaging, Technical Presentation. NEMB2016-6027
and diagnostic applications, the lack of quantification of the heat genera-
tion from plasmonic nanostructures has led to difficulties in predicting the Zilong Wu, Yuebing Zheng, The University of Texas at Austin, Aus-
therapeutic and diagnostic outcome and comparing the heat generating
capability for new plasmonic nanostructures. In this study, we quantitatively tin, TX, United States
measured the heat generation for GNPs, and examined the validity of the-
oretical predictions. Surprisingly, we showed that the agreement between Metasurfaces with moiré patterns, featured by a large number of component
experiment and theory is dependent on the type of GNP studied. Specifical- sets and high rotational symmetry, are promising for multi-functional appli-
ly, we found that the heat generation of gold nanospheres matches well with cations. With the hierarchical arrangements of plasmonic nanostructures of
theoretical prediction, while the measured heat generation for gold nanorod variable shapes and sizes, moiré metasurfaces can support multiple plas-
significantly deviates from theory, with only one third of the value predicted monic modes. We have developed high-throughput, cost-effective method
from theory at peak absorption. This then led to a surprising finding that the known as moiré nanosphere lithography (MNSL) for the moiré metasurfaces
polydispersity, i.e. the deviation of nanoparticle size and shape from nominal with multiple and tunable plasmonic modes. Furthermore, we have designed
value, significantly influences the optical properties of gold nanorod includ- a metal-insulator-metal (MIM) patch structure with a thin Au moiré metasur-
ing heat generation (>>70% reduction), but has very limited influence (<10%) face layer and an optically thick Au layer separated by a dielectric spacer
on gold nanospheres. We further demonstrate the importance of accounting layer. Benefiting from the combination of moiré patterns and field enhance-
for the polydispersity by an example comparing the photothermal absorp- ment from the MIM configuration, the moiré metasurface patch exhibits the
tion for gold nanosphere and nanorod with similar volume, leading to more strong absorption (>>95%) of incident light across a broad range of electro-
realistic predictions. This work suggests the significance of polydispersity magnetic spectrum, i.e, near-infrared (NIR) range from 900 nm to 1700 nm
in determining the plasmonic nanoparticle heat generation and provides a and mid-infrared (MIR) range (~5 um). Due to the broadband absorption in
method to quantitatively compare the heating capability for new plasmonic the NIR and MIR range, the moiré metasurface patch is proposed as a du-
nanostructures. al-functional substrate for spectroscopy and phototherapy.

11:50am Synthesis and Evaluation of Polymer Dynamic Organic 12:30pm Investigation Of The Use Of Ordered Nano-patterned
Spheres (PolyDOTS) Polycarbonate Films For Detecting Alzheimer`s Protein

Technical Presentation. NEMB2016-5952 Technical Presentation. NEMB2016-5985

Nicole Levi-Polyachenko, Wake Forest School of Medicine, Win- Sevde Altuntas, Fatih Buyukserin, TOBB University of Economics
ston-Salem, NC, United States, Elizabeth Graham, Wake Forest and Technology, ANKARA,Turkey
University health Sciences, Winston-Salem, NC, United States, Sne-
ha Kelkar, Wake Forest School of Medicine, Winston-Salem, NC, Today, Alzheimer’s disease is significantly more pronounced and it is
responsible for irreversible neural damage of cerebral cortex and hippo-
United States, Christopher MacNeill, Wake Forest University health campus. It seems possible to develop early diagnostic tools to decrease or
Sciences, Winston-Salem, NC, United States, Eleanor E. McCabe, annihilate harmful effects of the disease which seems to originate from the
Wake Forest School of Medicine, Winston-Salem, NC, United States Amyloid-β protein that accumulates in brain in the form plaques as dictated
by several literature reports. The basic problem of such a diagnostic tool for
Theranostic agents combine both a therapeutic agent and an imaging clinical or R & D study is the low amount of protein that cannot be detected
platform within the same material to simultaneously diagnose and treat dis- properly in body liquids such as blood, saliva or urine. To solve this problem,
eases, such as cancer. This report describes the development of donor-ac- tests like ELISA or PCR are proposed which are expensive, require special-
62 ceptor polymers into aqueously stable spherical nanoparticles that have ized personnel and can contain complex protocols.
the capacity to act as unique theranostic agents. We term these particles,
PolyDOTS, for polymer Dynamic Organic Theranostic Spheres. In this appli- Surface-enhanced Raman Spectroscopy (SERS) can potentially allow even