Page 63 - ASME_NEMB_2016_Program

TRACK 5 TRACK 5 Technical Program

single molecule detection in solutions or solid surfaces. In addition, SERS coated onto sensor surfaces and treated with oxgen-plasma with different
signal from a target molecule can be further increased by using nano pat- conditions. H9c2 cells with the same cell seeding density are added onto
terned surfaces when compared to smooth counterparts. Moreover, Raman sensor surface and admittance spectrum of the sensors is recorded during
signals are specific to molecules, so one can comment about content of a the cell attachment. The sensor’s sensitivity is evaluated by using spectral
sample. In this context, our study proposes to fabricate diagnostic test mod- signatures (i.e., peak frequency shift, peak admittance value shift) divided by
els that utilize Au-coated nano patterned polycarbonate (PC) surfaces mod- cell numbers at 6 hours after cell seeded onto the sensor surface. The effect
ified with Thioflavin - T to detect low concentrations of Amyloid-β protein of surface roughness and parylene C layer thickness on sensor’s sensitivity
in water and artificial saliva medium by the enhancement of protein SERS is investigated.
signal.
The nano patterned PC surface that was used to enhance SERS signal 5-3
was fabricated by using Anodic Alumina Membranes (AAM) as template. It
is possible to produce AAMs with different column structures and varying SELF-POWERED OR LOW ENERGY POWERED BIODEVICES
thicknesses depending on voltage and anodization time. After fabrication
process, the pore diameter of AAMs can be arranged with dilute acid solu- Hidalgo 11:30am - 1:00pm
tion treatment. In this study, two different columns structures were prepared.
After a surface modification to decrease their surface energy, AAMs were
treated with PC solution. Following the solvent evaporation, nano patterned 11:30am Highly efficient storage in Li3V2(PO4)3/C cathode Li-
PC films with tunable pillared structures were peeled off from the membrane ion batteries for self-powered biomedical devices
surface. The PC film was then modified with Au and Thioflavin-T for the de-
tection of Amyloid-β protein. The protein detection studies were conducted Keynote. NEMB2016-5970
first in water via this biosensor platform. Same measurements are currently
being conducted in artificial saliva to detect the presence of Amyloid-β
protein. SEM, SERS, FTIR, fluorescence microscopy and contact angle mea- Guozhong Cao, University of Washington, Seattle, WA, United
surements were carried out for the characterization of different surfaces and States, Xihui Nan, Beijing Institute of Nanoenergy and Nanosys-
further demonstration of the protein attachment. tems, Beijing, Beijing, China

This work was supported by The Scientific and Technological Research Lithium ion batteries are playing indispensible role in our modern life full of
Council of Turkey (TUBITAK) Grant No: 214Z167. portable electronics and in constant move. However, the advancement of
lithium ion batteries has lagged way behind electronics, in spite of extensive
12:50pm A Quartz Crystal Microbalance (QCM) sensor modified research efforts across research venues and industries. Search for better
with Parylene C coating for cellular monitoring electrode materials with both high energy and power densities as well as
excellent cyclic stability must consider (1) proper chemical reaction at the
interface between electrode and electrolyte: alloying, conversion reaction
Technical Presentation. NEMB2016-6014 and intercalation, (2) crystal structure with available space to accommodate
guest species such as spinel, olivine and layer structured crystals, (3) revers-
Jon Furniss, New York Institute of Technology, Old Westbury, NY, ible phase transition, and (4) manageable volume change. In addition, the
United States, Ioana voiculescu, City College of New York, New charge/discharge voltage and transport kinetics play determining roles in
York, NY, United States, Fang Li, New York Institute of Technology, the energy density and power density as well as the energy storage efficien-
New York, NY, United States cy of the resulting batteries.

Cell-based biosensors (CBBs), combined with living cells, have emerged as In this presentation, I will take a high performance Li3V2(PO4)3/C material
promising biotechnical tools in various fields, including drug development, synthesized by low-cost hydrothermal method followed with subsequent
medical diagnostics, cell-based therapy, and detection of chemical and bio- annealing treatment as an example to demonstrate the efficient storage of
logical agents. Among various CBB techniques, acoustic wave sensors have the high frequency pulse power generated by a triboelectric nanogenerator
attracted great attention due to their simple structure, compact size, excel- for biomedical applications. Not only does the Li3V2(PO4)3/C exhibit a dis-
lent time resolutions, high reliability, and sensitivity to the mechanical proper- charge capacity of 128 mAh g-1 at 1 C with excellent cyclic stability (capacity
ties of materials on the sensor’s surface. Especially, QCM sensor is the most retention is 90% after 1000 cycles at a rate of 5 C) in Li-ion battery, but also
commonly used acoustic wave device for cellular sensing due to its simple shows outstanding energy conversion efficiency (83.4%) compared with
structure and mature measurement technologies. Poly(chloro-para-xylylene) the most popular cathodic materials: LiFePO4 (74.4%), LiCoO2 (66.1%), and
or Parylene C is a material with a long history of use in the field of medical LiMn2O4 (73.6%) when it was charged by high frequency and large current
industry as a biocompatible encapsulant of implantable biomedical devices. electricity.
Recently, it has been gained particular interest as a material for in vitro cell
culture studies. It has been demonstrated that the surface properties of pa- 12:00pm Bubble-Pen Technique for Low-power Optical Manipu-
rylene C films, including the nanoroughness and wettability, could be tuned lation and Patterning of Cells and Biomolecules
through oxygen plasma treatments, which alters the cell adhesion to it. Stud-
ies have shown that the surface-treated parylene-C substrates exhibit adhe- Technical Presentation. NEMB2016-6041
sion levels comparable to commercially available tissue culture-treated poly-
styrene. In this paper, we investigate the parylene C modified QCM sensor
for dynamic and noninvasive cellular monitoring. The parylene-C layer coat- Xiaolei Peng, Yuebing Zheng, Linhan Lin, The University of Texas
ed on the sensor surface serves as a sensitivity-enhancing layer through at Austin, Austin, TX, United States
two aspects: (1) controlled surface nanoroughness increases the attachment
sites for cells, and the hydrophilic surface promotes cell adhesion; and (2) Versatile, non-invasive manipulation and patterning of cells and biomol-
the parylene C coating layer serves as an acoustic matching layer. Due to ecules play a critical role in early disease diagnosis, medicine, tissue
the big difference in acoustic impedance between quartz substrate and engineering, and fundamental studies in life sciences. Despite their high
cells, the acoustic waves transmitted from quartz substrate are reflected off. versatility, optical tweezers have remained challenging in the non-invasive
The parylene C layer inserted between the quartz substrate and cell layer operation. Herein, we develop a new method - bubble-pen technique - to
can promote acoustic wave propagation into cell layer, therefore enhancing non-invasively pattern cells and biomolecules on substrates using microbub-
sensor’s sensitivity. In this study, theoretically, transfer matrix method is used bles generated and controlled by low-power light beams. Briefly, a single 63
to study the effect of parylene C layer thickness on the sensor’s sensitivity. laser beam generates a microbubble at the interface of the solution and a
Experimentally, the parylene C films with three different thicknesses are plasmonic substrate via plasmon-enhanced photothermal effects. The mi-