Page 8 - An Identity Crisis
P. 8
MELTING AND CASTING GELS
May be performed in the early part of the class
period; for long classes, you may begin to run
the gels. Otherwise, store the gels in a ziploc bag
overnight in the refrigerator. ANALYZING THE GEL
WITH INSTASTAIN
If the gel must be stored overnight, or
RUNNING THE GEL you desire to resolve the bands more
Add the BlueVis running buffer to the electrophoresis for photodocumentation purposes,
chamber when you plan to start running the gel do not you may restain the gel with the
allow the gel to set overnight in the buffer. For classes included InstaStain sheets following the
with slower electrophoresis boxes (where the gel will procedure below:
take longer than the class period to run), optional
InstaStain sheets have been included to resolve the 1. Place the gel in a shallow plastic
DNA so that the gel may be stored overnight. container filled with distilled water.
2. Float the InstaStain sheet, blue side
down, on the water.
ANALYZING THE GEL IN REAL-TIME 3. Allow the gel to stain and destain
While the gel is running, and for several hours after overnight.
it has run, the DNA bands may be viewed with the
BlueVis dye present. BlueVis dye will fade with time,
and cannot be stored overnight.
8 THE MYSTERY OF LYLE AND LOUISE
