Page 51 - BJS vol. 35

Genetic Diversity Analysis and DNA Fingerprinting of Ten ..... Markers 43

Results of the present investigation revealed that the four SSR primers were able
to identify and classify ten sugarcane germplasm indicating genetic differences among
germplasm. Therefore, DNA fingerprinting and diversity analysis of all the germplasm as
well as entire germplasm collection should be done in order to determine their genetic
relationships among them.

Table 1. Parameters of primers sequences of four sugarcane microsatellite
primers from the International Sugarcane Microsatellite Consortium

Sequence G+C Content
Primer Code
(%)
SMC687CS Forward: -AGC CAT GCA GGC AGG CAT- 61.11
Reverse: -CGC ACA ATC TGC AAG TGC ATC A- 50.00

SMC334BS Forward: -CAA TTC TGA CCG TGC AAA GAT- 42.85
Reverse: -CGA TGA GCT TGA TTG CGA ATG- 47.61

SMC119CG Forward: -TTC ATC TCT AGC CTA CCC CAA- 47.61
Reverse: -AGC AGC CAT TTA CCC AGG A- 52.63

SMC766BS Forward: -TTA CTC GGC TGG GTT TTG TTC- 47.61
Reverse: -TAA GAA TCG TTC GCT CCA GC- 50.00

Table 2. Microsatellite primers with corresponding bands scored, their size range,
number of polymorphic bands, polymorphism and number of band per
germplasm together with germplasm distinguished in ten sugarcane
germplasm

Total
Size Number of Polymorph Number of Germplasm
Primer codes ranges number of polymorphic ism band per distinguished
bands
(bp) bands (%) germplasm (%)
scored

SMC687CS 90-172 19 1 5.26 1.90 20.00

SMC334BS 80-184 23 9 39.13 2.30 100.00

SMC119CG 89-124 26 3 11.54 2.60 30.00

SMC766BS 82-166 34 2 5.88 3.40 20.00
Total - 102 15 - 170
Average - 25.5 3.75 42.5